ArticleMethods (San Diego, Calif.)2010
Examining the contents of isolated Xenopus germinal vesicles.
Article in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 30 citations in OpenAlex.
- Molecular grammars of predicted intrinsically disordered regions that span the human proteome.Cell · 2026Article
- Micropipette aspiration reveals differential RNA-dependent viscoelasticity of nucleolar subcompartments.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Biochemical evidence that the whole compartment activity behavior of GAPDH differs between the cytoplasm and nucleus.PloS one · 2023Article
- Changes in Ploidy Drive Reproduction Transition and Genomic Diversity in a Polyploid Fish Complex.Molecular biology and evolution · 2022Article
- DNA-induced spatial entrapment of general transcription machinery can stabilize gene expression in a nondividing cell.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cold shock induces novel nuclear bodies in Xenopus oocytes.Experimental cell research · 2021Review
- Imaging the dynamics of transcription loops in living chromosomes.Chromosoma · 2018Article
- Lariat intronic RNAs in the cytoplasm of vertebrate cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte.Scientific reports · 2018Article
- Nuclear Actin in Development and Transcriptional Reprogramming.Frontiers in genetics · 2017Review
- Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders.Journal of visualized experiments : JoVE · 2016Article
- Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep.Scientific reports · 2015Article
- Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes.RNA (New York, N.Y.) · 2014Article
- Xenopus oocyte meiosis lacks spindle assembly checkpoint control.The Journal of cell biology · 2013Article
- Visualization of actin filaments and monomers in somatic cell nuclei.Molecular biology of the cell · 2013Article
- Induction of human lampbrush chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Association of modified cytosines and the methylated DNA-binding protein MeCP2 with distinctive structural domains of lampbrush chromatin.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Superresolution imaging of transcription units on newt lampbrush chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Three-dimensional organisation of RNA-processing machinery in avian growing oocyte nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Pearls are novel Cajal body-like structures in the Xenopus germinal vesicle that are dependent on RNA pol III transcription.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
One can manually isolate the giant oocyte nucleus or germinal vesicle (GV) of Xenopus from a living oocyte with nothing more complicated than jewelers' forceps and a dissecting microscope. Similarly, one can remove the nuclear envelope by hand and allow the lampbrush chromosomes and other nuclear organelles to spread on a microscope slide. After centrifugation, the nuclear contents adhere tightly to the slide, where they can be subjected to immunostaining or fluorescent in situ hybridization for visualization by conventional or confocal microscopy. Preparations of isolated GV contents reveal details of nuclear structure that are almost impossible to attain by more conventional techniques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.