ArticleMolecular and cellular biology1991
Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- The roles of phosphorylation of signaling proteins in the prognosis of acute myeloid leukemia.Pathology oncology research : POR · 2024Article
- Genetic perspective of retinoblastoma: From present to future.Indian journal of ophthalmology · 2016Review
- Aberrant DNA polymerase alpha is excluded from the nucleus by defective import and degradation in the nucleus.The Journal of biological chemistry · 2009Article
- KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association.Molecular vision · 2009Article
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- YB-1 promotes strand separation in vitro of duplex DNA containing either mispaired bases or cisplatin modifications, exhibits endonucleolytic activities and binds several DNA repair proteins.Nucleic acids research · 2004Article
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- Histone deacetylation of RB-responsive promoters: requisite for specific gene repression but dispensable for cell cycle inhibition.Molecular and cellular biology · 2003Article
- Distinct recruitment of E2F family members to specific E2F-binding sites mediates activation and repression of the E2F1 promoter.Oncogene · 2003Article
- CDP/Cux stimulates transcription from the DNA polymerase alpha gene promoter.Molecular and cellular biology · 2003Article
- Basal transcriptional regulation of human damage-specific DNA-binding protein genes DDB1 and DDB2 by Sp1, E2F, N-myc and NF1 elements.Nucleic acids research · 2003Article
- Structural basis for the recognition of the E2F transactivation domain by the retinoblastoma tumor suppressor.Genes & development · 2002Article
- Transcriptional coordination of the genes encoding catalytic (CK2alpha) and regulatory (CK2beta) subunits of human protein kinase CK2.Molecular and cellular biochemistry · 2001Article
- E2F mediates induction of the Sp1-controlled promoter of the human DNA polymerase epsilon B-subunit gene POLE2.Nucleic acids research · 2001Article
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- CREB/ATF-dependent repression of cyclin a by human T-cell leukemia virus type 1 Tax protein.Journal of virology · 2001Article
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- Cell cycle regulation of the tobacco ribonucleotide reductase small subunit gene is mediated by E2F-like elements.The Plant cell · 2000Article
- Thrombin activates a Y box-binding protein (DNA-binding protein B) in endothelial cells.The Journal of clinical investigation · 2000Article
- Dysregulation of cyclin E gene expression in human cytomegalovirus-infected cells requires viral early gene expression and is associated with changes in the Rb-related protein p130.Journal of virology · 2000Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We have investigated the DNA polymerase alpha promoter sequence requirements for the expression of a heterologous gene in actively cycling cells and following serum addition to serum-deprived cells. An 11.4-kb genomic clone that spans the 5' end of this gene and includes 1.62 kb of sequence upstream from the translation start site was isolated. The transcription start site was mapped at 46 +/- 1 nucleotides upstream from the translation start site. The upstream sequence is GC rich and lacks a TATA sequence but has a CCAAT sequence on the opposite strand. Analysis of a set of deletion constructs in transient transfection assays demonstrated that efficient expression of the reporter in cycling cells requires 248 bp of sequence upstream from the cap site. Clustered within these 248 nucleotides are sequences similar to consensus sequences for Sp1-, Ap1-, Ap2-, and E2F-binding sites. The CCAAT sequence and the potential E2F- and Ap1-binding sites are shown to be protected from DNase I digestion by partially purified nuclear proteins. The DNA polymerase alpha promoter can confer upon the reporter an appropriate, late response to serum addition. No single sequence element could be shown to confer serum inducibility. Rather, multiple sequence elements appear to mediate the full serum response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.