ArticleStem cell reviews and reports2009
Cell lines derived from human parthenogenetic embryos can display aberrant centriole distribution and altered expression levels of mitotic spindle check-point transcripts.
Article in Stem cell reviews and reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- "Biomechanical Signaling in Oocytes and Parthenogenetic Cells".Frontiers in cell and developmental biology · 2021Review
- Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts.Stem cell reviews and reports · 2019Article
- Inactivation of PLK4-STIL Module Prevents Self-Renewal and Triggers p53-Dependent Differentiation in Human Pluripotent Stem Cells.Stem cell reports · 2018Article
- 5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts.Scientific reports · 2016Article
- Pluripotent stem cells in disease modelling and drug discovery.Nature reviews. Molecular cell biology · 2016Review
- Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences.Cell and tissue research · 2016Review
- Human Parthenogenetic Embryonic Stem Cell-Derived Neural Stem Cells Express HLA-G and Show Unique Resistance to NK Cell-Mediated Killing.Molecular medicine (Cambridge, Mass.) · 2015Article
- Morphological and molecular changes of human granulosa cells exposed to 5-azacytidine and addressed toward muscular differentiation.Stem cell reviews and reports · 2014Article
- Brief demethylation step allows the conversion of adult human skin fibroblasts into insulin-secreting cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Centrosome amplification and chromosomal instability in human and animal parthenogenetic cell lines.Stem cell reviews and reports · 2012Article
- Article
- Global analysis of parental imprinting in human parthenogenetic induced pluripotent stem cells.Nature structural & molecular biology · 2011Article
- Use of parthenogenetic activation of human oocytes as an experimental model for evaluation of polar body based PGD assay performance.Journal of assisted reproduction and genetics · 2011Article
- Derivation of rhesus monkey parthenogenetic embryonic stem cells and its microRNA signature.PloS one · 2011Article
Corrections and comments
- Expression of concern
Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human parthenogenetic embryos have recently been proposed as an alternative, less controversial source of embryonic stem cell (ESC) lines; however many aspects related to the biology of parthenogenetic embryos and parthenogenetic derived cell lines still need to be elucidated. We present here results on human cell lines (HP1 and HP3) derived from blastocysts obtained by oocyte parthenogenetic activation. Cell lines showed typical ESC morphology, expressed Oct-4, Nanog, Sox-2, Rex-1, alkaline phosphatase, SSEA-4, TRA 1-81 and had high telomerase activity. Expression of genes specific for different embryonic germ layers was detected from HP cells differentiated upon embryoid body (EBs) formation. Furthermore, when cultured in appropriate conditions, HP cell lines were able to differentiate into mature cell types of the neural and hematopoietic lineages. However, the injection of undifferentiated HP cells in immunodeficient mice resulted either in poor differentiation or in tumour formation with the morphological characteristics of myofibrosarcomas. Further analysis of HP cells indicated aberrant levels of molecules related to spindle formation as well as the presence of an abnormal number of centrioles and autophagic activity. Our results confirm and extend the notion that human parthenogenetic stem cells can be derived and can differentiate in mature cell types, but also highlight the possibility that, alteration of the proliferation mechanisms may occur in these cells, suggesting great caution if a therapeutic use of this kind of stem cells is considered.
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