ArticleMethods in molecular biology (Clifton, N.J.)2009
iTRAQ-based shotgun neuroproteomics.
Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Methodological Aspects of μLC-MS/MS for Wide-Scale Proteomic Analysis of Anthracycline-Induced Cardiomyopathy.ACS omega · 2025Article
- Advances in neuroproteomics for neurotrauma: unraveling insights for personalized medicine and future prospects.Frontiers in neurology · 2023Review
- Identification of 14-3-3 epsilon as a regulator of the neural apoptotic pathway for chronic-stress-induced depression.iScience · 2021Article
- Medial prefrontal cortex exacerbates gastric dysfunction of rats upon restraint water‑immersion stress.Molecular medicine reports · 2019Article
- Sox11 promotes head and neck cancer progression via the regulation of SDCCAG8.Journal of experimental & clinical cancer research : CR · 2019Article
- iTRAQ-based quantitative proteomic analysis provides insight for molecular mechanism of neuroticism.Clinical proteomics · 2019Article
- Plasma proteomics reveals coagulation, inflammation, and metabolic shifts in H-type hypertension patients with and without acute ischemic stroke.Oncotarget · 2017Article
- Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats.The international journal of neuropsychopharmacology · 2015Article
- Isobaric labeling-based relative quantification in shotgun proteomics.Journal of proteome research · 2014Review
- Article
- Chronic exposure to light reverses the effect of maternal separation on proteins in the prefrontal cortex.Journal of molecular neuroscience : MN · 2013Article
- Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.Stem cell research & therapy · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Shotgun proteomics involves the analysis of peptides obtained by enzymatic digestions of proteins and subsequent identification via tandem mass spectrometry. This approach is an effective method for studying global protein expression in neuronal systems. The method described here is a quantitative shotgun neuroproteomics method using amine-specific isobaric tags for a relative and absolute quantitation (iTRAQ)-based workflow. We will provide the technical details for sample preparation, two-dimensional liquid chromatography, tandem mass spectrometry, database search, and statistical analysis to identify differentially expressed proteins. We will use a recent study on a rat model of multiple sclerosis, experimental autoimmune encephalomyelitis to illustrate the successful application of this method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.