ArticleJournal of gastroenterology2010
Intravital imaging of DSS-induced cecal mucosal damage in GFP-transgenic mice using two-photon microscopy.
Article in Journal of gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Intravital imaging of translocated bacteria via fluorogenic labeling of gut microbiota in situ.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Intravital imaging of immune responses in intestinal inflammation.Inflammation and regeneration · 2023Review
- Regulation of the Gut Microbiota and Inflammation by β-Caryophyllene Extracted from Cloves in a Dextran Sulfate Sodium-Induced Colitis Mouse Model.Molecules (Basel, Switzerland) · 2022Article
- Gut Microbiota Regulate Gut-Lung Axis Inflammatory Responses by Mediating ILC2 Compartmental Migration.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Live Intravital Intestine with Blood Flow Visualization in Neonatal Mice Using Two-photon Laser Scanning Microscopy.Bio-protocol · 2021Article
- Method for Acute Intravital Imaging of the Large Intestine in Live Mice.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Activation of Wnt signaling by amniotic fluid stem cell-derived extracellular vesicles attenuates intestinal injury in experimental necrotizing enterocolitis.Cell death & disease · 2020Article
- Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model.JCI insight · 2018Article
- The possibility of using shogaol for treatment of ulcerative colitis.Iranian journal of basic medical sciences · 2018Article
- Regulatory effects of glycyrrhizae radix extract on DSS-induced ulcerative colitis.BMC complementary and alternative medicine · 2016Article
- Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy.Oncology letters · 2016Article
- Long term intravital multiphoton microscopy imaging of immune cells in healthy and diseased liver using CXCR6.Gfp reporter mice.Journal of visualized experiments : JoVE · 2015Article
- In vivo optical pathology of paclitaxel efficacy on the peritoneal metastatic xenograft model of gastric cancer using two-photon laser scanning microscopy.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2015Article
- Visualization of in vivo thromboprophylactic and thrombolytic efficacy of enoxaparin in laser-induced vascular endothelial injury model using multiphoton microscopy.American journal of translational research · 2015Article
- Dynamic pathology for circulating free DNA in a dextran sodium sulfate colitis mouse model.Pediatric surgery international · 2014Article
- Advanced Motion Compensation Methods for Intravital Optical Microscopy.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society · 2014Article
- In vivo characterization of neutrophil extracellular traps in various organs of a murine sepsis model.PloS one · 2014Article
- In vivo optical imaging of cancer metastasis using multiphoton microscopy: a short review.American journal of translational research · 2014Review
- Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Caught in the act: revealing the metastatic process by live imaging.Disease models & mechanisms · 2013Review
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTwo-photon laser-scanning microscopy (TPLSM) is a powerful diagnostic tool for real-time, high-resolution structural imaging. However, obtaining high-quality in vivo TPLSM images of intra-abdominal organs remains technically challenging. MATERIALS AND
methodsAn organ-stabilizing system was applied to high-quality TPLSM imaging. Real-time imaging of visceral organs, such as the liver, spleen, kidney and intestine, of transgenic green fluorescent protein (GFP) mice was performed in vivo using TPLSM. The bacterial translocation model using dextran sodium sulfate (DSS)-induced colitis was also investigated in prepared GFP mice following simple surgery. This allowed the capture of morphological real images using in vivo TPLSM. Immunohistochemical analysis of ZO-1 was performed to support the morphological findings of TPLSM. RESULTS AND
conclusionsWe established an organ-stabilizing system to evaluate the real-time imaging of visceral organs in actin-GFP transgenic mice using in vivo TPLSM. DSS-induced colitis showed irregularity of crypt architecture, disappearance of crypts, inflammatory cell infiltration and increased rolling of white blood cells along the vasculature. In addition, the intercellular distance of mucosal cells in the crypt and vascular endothelial cells in the intestinal wall was increased in the intestinal mucosa during DSS colitis. In DSS colitis, there was remarkable loss of mucosal and vascular endothelial ZO-1 expression, as could be seen by a decrease in ZO-1 staining. In conclusion, our observations suggested the possibility that our TPLSM imaging system can be used to clarify the pathophysiological changes in various diseases using longitudinal studies of microscopic changes in the same animal over long periods of time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.