ArticleInternational journal of clinical and experimental medicine2009
Acid stress response of a mycobacterial proteome: insight from a gene ontology analysis.
Article in International journal of clinical and experimental medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 14 citations in OpenAlex.
- Metabolomics Studies To Decipher Stress Responses in Mycobacterium smegmatis Point to a Putative Pathway of Methylated Amine Biosynthesis.Journal of bacteriology · 2019Article
- PknG supports mycobacterial adaptation in acidic environment.Molecular and cellular biochemistry · 2018Article
- Latent tuberculosis: what the host "sees"?Immunologic research · 2011Review
- Label-free proteomics and systems biology analysis of mycobacterial phagosomes in dendritic cells and macrophages.Journal of proteome research · 2011Article
- Assigning significance in label-free quantitative proteomics to include single-peptide-hit proteins with low replicates.International journal of proteomics · 2010Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acidity in vesicles of macrophages is a general signal that bacteria respond to during infection. Mycobacteria are particularly capable of resisting the acidification in macrophages that engulf the bacteria. In this work, we used label-free quantitative proteomics to study the Mycobacterium smegmatis proteome under acid stress so as to gain an insight into the acidic adaptation in mycobacteria. We quantified 1032 proteins. With a 3-fold change threshold, 20 and 52 proteins were found regulated at false discovery rates of 5% and 14% respectively. We performed a systems analysis based on gene ontology for the global proteome expression profile. We found that the most significant changes induced by the acid stress include a downregulation of transmembrane transporter activity and an upregulation of enzymes involved in fatty acid metabolism. The results suggest that reduced transmembrane transport and increased fatty acid metabolism probably contribute to or associate with acid tolerance in mycobacteria.
Indexed as
Identifiers
20057975PMC2802051W63297889What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.