ArticleThe EMBO journal2010
GPI anchoring facilitates propagation and spread of misfolded Sup35 aggregates in mammalian cells.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Membrane composition and lipid to protein ratio modulate amyloid kinetics of yeast prion protein.RSC chemical biology · 2021Article
- Aerosol Delivery of Synthetic mRNA to Vaginal Mucosa Leads to Durable Expression of Broadly Neutralizing Antibodies against HIV.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- PrP Knockout Cells Expressing Transmembrane PrP Resist Prion Infection.Journal of virology · 2017Article
- Efficient uptake and dissemination of scrapie prion protein by astrocytes and fibroblasts from adult hamster brain.PloS one · 2015Article
- Glycosylphosphatidylinositol anchoring directs the assembly of Sup35NM protein into non-fibrillar, membrane-bound aggregates.The Journal of biological chemistry · 2014Article
- Live imaging of prions reveals nascent PrPSc in cell-surface, raft-associated amyloid strings and webs.The Journal of cell biology · 2014Article
- Caenorhabditis elegans as a model system for studying non-cell-autonomous mechanisms in protein-misfolding diseases.Disease models & mechanisms · 2014Review
- Cell-to-cell propagation of infectious cytosolic protein aggregates.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Prions Ex Vivo: What Cell Culture Models Tell Us about Infectious Proteins.International journal of cell biology · 2013Review
- Prions and the potential transmissibility of protein misfolding diseases.Annual review of microbiology · 2013Review
- Seeding plaques in Alzheimer's disease.Journal of neurochemistry · 2012Article
- The septin cytoskeleton facilitates membrane retraction during motility and blebbing.The Journal of cell biology · 2012Article
- Dual conformation of H2H3 domain of prion protein in mammalian cells.The Journal of biological chemistry · 2011Article
- Amyloid-like aggregates of the yeast prion protein ure2 enter vertebrate cells by specific endocytotic pathways and induce apoptosis.PloS one · 2010Article
- Emergence and natural selection of drug-resistant prions.Molecular bioSystems · 2010Review
- Prion-like disorders: blurring the divide between transmissibility and infectivity.Journal of cell science · 2010Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Prion diseases differ from other amyloid-associated protein misfolding diseases (e.g. Alzheimer's) because they are naturally transmitted between individuals and involve spread of protein aggregation between tissues. Factors underlying these features of prion diseases are poorly understood. Of all protein misfolding disorders, only prion diseases involve the misfolding of a glycosylphosphatidylinositol (GPI)-anchored protein. To test whether GPI anchoring can modulate the propagation and spread of protein aggregates, a GPI-anchored version of the amyloidogenic yeast protein Sup35NM (Sup35GPI) was expressed in neuronal cells. Treatment of cells with Sup35NM fibrils induced the GPI anchor-dependent formation of self-propagating, detergent-insoluble, protease-resistant, prion-like aggregates of Sup35GPI. Live-cell imaging showed intercellular spread of Sup35GPI aggregation to involve contact between aggregate-positive and aggregate-negative cells and transfer of Sup35GPI from aggregate-positive cells. These data demonstrate GPI anchoring facilitates the propagation and spread of protein aggregation and thus may enhance the transmissibility and pathogenesis of prion diseases relative to other protein misfolding diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.