ArticleBMC plant biology2010
Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers.
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Who cites it
152 citing papers in PubMed, 313 citations in OpenAlex.
- Article
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- Suppressor activity against cosuppression conferred by the petunia vein clearing virus genome.Transgenic research · 2026Article
- Transketolase-mediated erythrose-4-phosphate provides an essential source for anthocyanin biosynthesis in petunia.Horticulture research · 2025Article
- Validation of a bitmap of genes involved in cherry fruit cracking by digital PCR and qPCR, suitable for plant breeding.Scientific reports · 2025Article
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- Genome-Wide Analysis of GRETCHEN HAGEN3 Genes and Characterization of IAA-Amido Synthetase GenePlants (Basel, Switzerland) · 2025Article
- Selection and validation of reference genes in alfalfa based on transcriptome sequence data.Scientific reports · 2025Article
- Genome-wide identification and functional characterization of the two-component system gene family in petunia reveals roles in hormone signaling and stress response.Frontiers in plant science · 2025Article
- Transcriptomic and hormonal dynamics in relation to adventitious rooting of two parentalFrontiers in plant science · 2025Article
- CRISPR/Cas9-mediated mutagenesis of FT/TFL1 in petunia improves plant architecture and early flowering.Plant molecular biology · 2024Article
- Exploring laccase: a sustainable enzymatic solution for the paper recycling domain.Archives of microbiology · 2024Review
- Abscisic acid-induced transcription factor PsMYB306 negatively regulates tree peony bud dormancy release.Plant physiology · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Comprehensive Analyses of FourPlants (Basel, Switzerland) · 2024Article
- Transcriptome-Based Identification of the Optimal Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Analyses of Lingonberry Fruits throughout the Growth Cycle.Plants (Basel, Switzerland) · 2023Article
- Selection and validation of reliable reference genes for quantitative real-time PCR in Barnyard millet (Echinochloa spp.) under varied abiotic stress conditions.Scientific reports · 2023Article
- Hormonal Signaling during dPCD: Cytokinin as the Determinant of RNase-Based Self-Incompatibility in Solanaceae.Biomolecules · 2023Article
- Functional characterization of three TERMINAL FLOWER 1-like genes from Platanus acerifolia.Plant cell reports · 2023Article
- Drought and Oxidative Stress in Flax (Antioxidants (Basel, Switzerland) · 2023Article
92 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIdentification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition tested as well as the methods used for RNA purification and analysis.
resultsIn an effort to identify endogenous genes meeting these criteria nine reference genes (RG) were tested in two Petunia lines (Mitchell and V30). Growth conditions differed in Mitchell and V30, and different methods were used for RNA isolation and analysis. Four different software tools were employed to analyze the data. We merged the four outputs by means of a non-weighted unsupervised rank aggregation method. The genes identified as optimal for transcriptomic analysis of Mitchell and V30 were EF1alpha in Mitchell and CYP in V30, whereas the least suitable gene was GAPDH in both lines.
conclusionsThe least adequate gene turned out to be GAPDH indicating that it should be rejected as reference gene in Petunia. The absence of correspondence of the best-suited genes suggests that assessing reference gene stability is needed when performing normalization of data from transcriptomic analysis of flower and leaf development.
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