ArticleMolecular genetics and genomics : MGG2010
Mediator subunits and histone methyltransferase Set2 contribute to Ino2-dependent transcriptional activation of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.
Article in Molecular genetics and genomics : MGG, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Structural Analysis of Ino2p/Ino4p Mutual Interactions and Their Binding Interface with Promoter DNA.International journal of molecular sciences · 2022Article
- Article
- The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator ofPeerJ · 2017Article
- The Mediator Complex and Lipid Metabolism.Journal of biochemical and pharmacological research · 2013Article
- SCS3 and YFT2 link transcription of phospholipid biosynthetic genes to ER stress and the UPR.PLoS genetics · 2012Article
- Novel role for mediator complex subunit Srb5/Med18 in termination of transcription.The Journal of biological chemistry · 2011Article
- MED19 promotes proliferation and tumorigenesis of lung cancer.Molecular and cellular biochemistry · 2011Article
- Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation.Molecular and cellular biology · 2010Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
To activate eukaryotic genes, several pathways which modify chromatin and recruit general factors of the transcriptional machinery are utilized. We investigated the factors required for activation of yeast phospholipid biosynthetic genes, depending on activator protein Ino2 which binds to the inositol/choline-responsive element (ICRE) upstream promoter motif together with its partner protein Ino4. We used a set of 15 strains each defective for one of the non essential subunits of yeast mediator complex and identified med2, med3, med15, med18 and med19 as impaired for inositol biosynthesis. In these mutants, ICRE-dependent gene activation was reduced to 13-22% of the wild-type level. We also demonstrate synthetic growth and activation defects among mediator mutants and mutants lacking defined histone modifications (snf1, gcn5) and transcriptional coactivators (sub1). Analysis of mutants defective for histone methylation (set1, set2 and dot1) and demethylation (jhd1, jhd2, gis1, rph1 and ecm5) revealed the importance of the H3 Lys36-specific Set2 methyltransferase for ICRE-dependent gene expression. Although defined mediator subunits are critical for gene activation, we could not detect their interaction with Ino2. In contrast, Ino2 directly binds to the Set2 histone methyltransferase. Mapping of interaction domains revealed the importance of the SET core domain which was necessary and sufficient for binding Ino2.
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