ArticleActa crystallographica. Section F, Structural biology and crystallization communications2009
Crystallization and preliminary crystallographic characterization of glutamine synthetase from Medicago truncatula.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity.Medical sciences (Basel, Switzerland) · 2022Review
- New isoforms and assembly of glutamine synthetase in the leaf of wheat (Triticum aestivum L.).Journal of experimental botany · 2015Article
- Redundancy and metabolic function of the glutamine synthetase gene family in poplar.BMC plant biology · 2015Article
- Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme.Frontiers in plant science · 2015Review
- The structures of cytosolic and plastid-located glutamine synthetases from Medicago truncatula reveal a common and dynamic architecture.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Novel aspects of glutamine synthetase (GS) regulation revealed by a detailed expression analysis of the entire GS gene family of Medicago truncatula under different physiological conditions.BMC plant biology · 2013Article
- Possible role of glutamine synthetase in the NO signaling response in root nodules by contributing to the antioxidant defenses.Frontiers in plant science · 2013Article
- Glutamine synthetase in legumes: recent advances in enzyme structure and functional genomics.International journal of molecular sciences · 2012Review
- Glutamine synthetase is a molecular target of nitric oxide in root nodules of Medicago truncatula and is regulated by tyrosine nitration.Plant physiology · 2011Article
- The glutamine synthetase gene family in Populus.BMC plant biology · 2011Article
- Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds.BMC plant biology · 2010Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The condensation of ammonium and glutamate into glutamine catalyzed by glutamine synthetase (GS) is a fundamental step in nitrogen metabolism in all kingdoms of life. In plants, this is preceded by the reduction of inorganic nitrogen to an ammonium ion and therefore effectively articulates nitrogen fixation and metabolism. Although the three-dimensional structure of the dodecameric bacterial GS was determined quite some time ago, the quaternary architecture of the plant enzyme has long been assumed to be octameric, mostly on the basis of low-resolution electron-microscopy studies. Recently, the crystallographic structure of a monocotyledonous plant GS was reported that revealed a homodecameric organization. In order to unambiguously establish the quaternary architecture of GS from dicotyledonous plants, GS1a from the model legume Medicago truncatula was overexpressed, purified and crystallized. The collection of synchrotron diffraction data to 2.35 A resolution allowed the determination of the three-dimensional structure of this enzyme by molecular replacement.
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