ArticleInvestigative ophthalmology & visual science2010
Responses of cultured human keratocytes and myofibroblasts to ethyl pyruvate: a microarray analysis of gene expression.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Multitargeted Neuroprotective Strategies of Ethyl Pyruvate: From Molecular Mechanisms to Clinical Perspectives in CNS Disorders.Biomolecules & therapeutics · 2026Review
- Pathological Interplay of ROS With Myofibroblasts: An Impediment to Corneal Restitution.Oxidative medicine and cellular longevity · 2026Review
- Exogenous Pyruvate in Defense Against Human-Exposure Toxicants: A Review of In Vitro and In Vivo Evidence.International journal of molecular sciences · 2025Review
- RNA-Seq Analysis Unraveling Novel Genes and Pathways Influencing Corneal Wound Healing.Investigative ophthalmology & visual science · 2024Article
- Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids.International journal of molecular sciences · 2024Article
- Transcriptome from opaque cornea of Fanconi anemia patient uncovers fibrosis and two connected players.Molecular genetics and metabolism reports · 2021Article
- Nrf2 signaling and inflammation are key events in physical plasma-spurred wound healing.Theranostics · 2019Article
- Featured Article: Pyruvate preserves antiglycation defenses in porcine brain after cardiac arrest.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Nrf2, a Potential Therapeutic Target against Oxidative Stress in Corneal Diseases.Oxidative medicine and cellular longevity · 2017Review
- A pilot exome-wide association study of age-related cataract in Koreans.Journal of biomedical research · 2016Article
- Endogenous sulfur dioxide alleviates collagen remodeling via inhibiting TGF-β/Smad pathway in vascular smooth muscle cells.Scientific reports · 2016Article
- Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells.Molecular vision · 2013Article
- Ethyl pyruvate ameliorates endotoxin-induced corneal inflammation.Investigative ophthalmology & visual science · 2012Article
- Critical role of Klf5 in regulating gene expression during post-eyelid opening maturation of mouse corneas.PloS one · 2012Article
- Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.Investigative ophthalmology & visual science · 2011Article
- Preventive effects of ethyl pyruvate on endotoxin-induced uveitis in rats.Investigative ophthalmology & visual science · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeEthyl pyruvate (EP) has pharmacologic effects that remediate cellular stress. In the organ-cultured murine lens, EP ameliorates oxidative stress, and in a rat cataract model, it attenuates cataract formation. However, corneal responses to EP have not been elucidated. In this study, the potential of EP as a therapeutic agent in corneal wound healing was determined by examining its effects on the transition of quiescent corneal stromal keratocytes into contractile myofibroblasts.
methodsThree independent preparations of cultured human keratocytes were treated with TGF-beta1, to elicit a phenotypic transition to myofibroblasts in the presence or absence of 10 or 15 mM EP. Gene expression profiles of the 12 samples (keratocytes +/- EP +/- TGF-beta1 for three preparations) were produced by using gene microarrays.
resultsTGF-beta1-driven twofold changes in at least two of three experiments defined a group of 1961 genes. Genes showing twofold modulation by EP in at least two experiments appeared exclusively in myofibroblasts (857 genes), exclusively in keratocytes (409 genes), or in both phenotypes (252 genes). Analysis of these three EP-modulated groups showed that EP (1) inhibited myofibroblast proliferation with concomitant modulation of some cell cycle genes, (2) augmented the NRF2-mediated antioxidant response in both keratocytes and myofibroblasts, and (3) modified the TGF-beta1-driven transition of keratocytes to myofibroblasts by inhibiting the upregulation of a subset of profibrotic genes.
conclusionsThese EP-induced phenotypic changes in myofibroblasts indicate the potential of EP as a therapeutic agent in corneal wound healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.