ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2010
Glycine release from radial cells modulates the spontaneous activity and its propagation during early spinal cord development.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 76 citations in OpenAlex.
- Role of the Glycine Transporter GlyT2 in the Neuronal Differentiation of PC12 Cells.International journal of molecular sciences · 2026Article
- Article
- Spontaneous activity in whisker-innervating region of neonatal mouse trigeminal ganglion.Scientific reports · 2022Article
- Glycine-induced NMDA receptor internalization provides neuroprotection and preserves vasculature following ischemic stroke.iScience · 2022Article
- Glycine neurotransmission: Its role in development.Frontiers in neuroscience · 2022Review
- Glycine encephalopathy.The Egyptian journal of neurology, psychiatry and neurosurgery · 2022Review
- Loss, Gain and Altered Function of GlyR α2 Subunit Mutations in Neurodevelopmental Disorders.Frontiers in molecular neuroscience · 2022Article
- Article
- Glycine Regulates Neural Stem Cell Proliferation During DevelopmentFrontiers in molecular neuroscience · 2019Article
- Early Actions of Neurotransmitters During Cortex Development and Maturation of Reprogrammed Neurons.Frontiers in synaptic neuroscience · 2019Review
- Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature.Nature communications · 2018Article
- Persistent Sodium Current Drives Excitability of Immature Renshaw Cells in Early Embryonic Spinal Networks.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Glycine Promotes the Survival of a Subpopulation of Neural Stem Cells.Frontiers in cell and developmental biology · 2018Article
- Microcircuit formation following transplantation of mouse embryonic stem cell-derived neurons in peripheral nerve.Journal of neurophysiology · 2017Article
- Spontaneous and Evoked Activity from Murine Ventral Horn Cultures on Microelectrode Arrays.Frontiers in cellular neuroscience · 2017Article
- Structure-Function Analysis of the GlyR α2 Subunit Autism Mutation p.R323L Reveals a Gain-of-Function.Frontiers in molecular neuroscience · 2017Article
- Genetic and functional analyses demonstrate a role for abnormal glycinergic signaling in autism.Molecular psychiatry · 2016Article
- Development of Spontaneous Activity in the Avian Hindbrain.Frontiers in neural circuits · 2016Review
- Regulation of Spontaneous Propagating Waves in the Embryonic Mouse Brainstem.Frontiers in neural circuits · 2016Article
- Electrical maturation of spinal neurons in the human fetus: comparison of ventral and dorsal horn.Journal of neurophysiology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhythmic electrical activity is a hallmark of the developing embryonic CNS and is required for proper development in addition to genetic programs. Neurotransmitter release contributes to the genesis of this activity. In the mouse spinal cord, this rhythmic activity occurs after embryonic day 11.5 (E11.5) as waves spreading along the entire cord. At E12.5, blocking glycine receptors alters the propagation of the rhythmic activity, but the cellular source of the glycine receptor agonist, the release mechanisms, and its function remain obscure. At this early stage, the presence of synaptic activity even remains unexplored. Using isolated embryonic spinal cord preparations and whole-cell patch-clamp recordings of identified motoneurons, we find that the first synaptic activity develops at E12.5 and is mainly GABAergic. Using a multiple approach including direct measurement of neurotransmitter release (i.e., outside-out sniffer technique), we also show that, between E12.5 and E14.5, the main source of glycine in the embryonic spinal cord is radial cell progenitors, also known to be involved in neuronal migration. We then demonstrate that radial cells can release glycine during synaptogenesis. This spontaneous non-neuronal glycine release can also be evoked by mechanical stimuli and occurs through volume-sensitive chloride channels. Finally, we find that basal glycine release upregulates the propagating spontaneous rhythmic activity by depolarizing immature neurons and by increasing membrane potential fluctuations. Our data raise the question of a new role of radial cells as secretory cells involved in the modulation of the spontaneous electrical activity of embryonic neuronal networks.
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