ArticleJournal of clinical microbiology2010
Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Periprosthetic Joint Infection Caused byArthroplasty today · 2026Article
- Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis.Scientific reports · 2023Article
- First seroprevalence and molecular identification report ofVeterinary world · 2023Article
- Assays for Identification and Differentiation ofMicroorganisms · 2022Review
- High-Resolution Melting PCR as Rapid Genotyping Tool forMicroorganisms · 2022Article
- MALDI-TOF mass spectrometry and high-resolution melting PCR for the identification of Mycoplasma bovis isolates.BMC veterinary research · 2021Article
- Direct detection of Corynebacterium striatum, Corynebacterium propinquum, and Corynebacterium simulans in sputum samples by high-resolution melt curve analysis.BMC infectious diseases · 2021Article
- Canine Brucellosis: An Update.Frontiers in veterinary science · 2021Review
- Molecular characterization of Brucella species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania.Veterinary medicine and science · 2020Article
- Brucellosis in China: history, progress and challenge.Infectious diseases of poverty · 2020Article
- Three-reaction high-resolution melting assay for rapid differentiation of Mycobacterium tuberculosis complex members.MicrobiologyOpen · 2019Article
- Multiplex Real-Time PCR with HRM for Detection ofFood technology and biotechnology · 2019Article
- Development of a new High Resolution Melting (HRM) assay for identification and differentiation of Mycobacterium tuberculosis complex samples.Scientific reports · 2019Article
- Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis SmoothFrontiers in veterinary science · 2019Article
- Pediatric brucellosis. An update review for the new millennium.Saudi medical journal · 2018Review
- Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood.Viruses · 2017Article
- Brucella neotomae Infection in Humans, Costa Rica.Emerging infectious diseases · 2017Article
- Article
- Differential diagnosis of Brucella abortus by real-time PCR based on a single-nucleotide polymorphisms.The Journal of veterinary medical science · 2016Article
- Detection and discrimination of two Brucella species by multiplex real-time PCR and high-resolution melt analysis curve from human blood and comparison of results using RFLP.Iranian journal of basic medical sciences · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Definitive identification of Brucella species remains a challenge due to the high degree of genetic homology shared within the genus. We report the development of a molecular technique which utilizes real-time PCR followed by high-resolution melt (HRM) curve analysis to reliably type members of this genus. Using a panel of seven primer sets, we tested 153 Brucella spp. isolates with >99% accuracy compared to traditional techniques. This assay provides a useful diagnostic tool that can rapidly type Brucella isolates and has the potential to detect novel species. This approach may also prove helpful for clinical, epidemiological and veterinary investigations.
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