ArticleJournal of clinical microbiology2010
Multiplex-PCR method for species identification of coagulase-positive staphylococci.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 192 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
192 citing papers in PubMed, 435 citations in OpenAlex.
- Screening Equine Skin and Mucous Membranes for Carriage of Coagulase-Positive Staphylococci.Veterinary dermatology · 2026Article
- Companion animals as potentially important reservoir of methicillin-resistant and virulent strains of coagulase-positive Staphylococcus spp.BMC veterinary research · 2026Article
- Phytochemical Characterization and Antibiofilm Efficacy ofAntibiotics (Basel, Switzerland) · 2026Article
- Activity of thieves-type protective blends and their individual components (cinnamon, clove, rosemary, eucalyptus and lemon essential oils) against Staphylococcus pseudintermedius strains isolated from canine otitis externa.BMC veterinary research · 2026Article
- Mammaliicoccus sciuri as a sentinel for antimicrobial resistance and virulence: genomic epidemiology, transmission dynamics, and control in the one health era.Veterinary research communications · 2026Review
- IntratumoralAnimals : an open access journal from MDPI · 2026Article
- Perinatal Occurrence and Epidemiological Significance ofAnimals : an open access journal from MDPI · 2026Article
- Genetic and phenotypic identities of Staphylococcus coagulans isolated from pustules of dogs with superficial bacterial folliculitis.BMC veterinary research · 2026Article
- Emergence of methicillin resistantFrontiers in veterinary science · 2026Article
- Diversity among isolates of Staphylococcus intermedius group from Pacific marine mammals of Southern California.PloS one · 2026Article
- Tetracycline Resistance Among Canine Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- False positive results obtained with latex agglutination identification of Staphylococcus aureus caused by Macrococcus caseolyticus and Mammaliicoccus fleurettii.Scientific reports · 2025Article
- Molecular epidemiology and environmental persistence of methicillin-resistant coagulase-positiveVeterinary world · 2025Article
- Molecular detection and characterization of methicillin-resistantOne health (Amsterdam, Netherlands) · 2025Article
- Nationwide surveillance and molecular characterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats in South Korea.Scientific reports · 2025Article
- Isolation and Molecular Characterization of Three Staphylococcus pseudintermedius Strains from Dogs and Humans in Egypt.Current microbiology · 2025Article
- Synergistic Antibacterial Effect of Eugenol and Biogenic Silver Nanoparticles onMolecules (Basel, Switzerland) · 2025Article
- Article
- Antibacterial Activity of Clove Essential Oil (Pathogens (Basel, Switzerland) · 2025Article
- Monolaurin inhibits antibiotic-resistant Staphylococcus aureus in patients with atopic dermatitis.Scientific reports · 2025Article
132 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene locus. To evaluate sensitivity and specificity, we used this M-PCR method on 374 staphylococcal strains that had been previously identified to the species level by an hsp60 sequencing approach. We could successfully distinguish between S. aureus, S. hyicus, S. schleiferi, S. intermedius, S. pseudintermedius, and S. delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.