ArticleNucleic acids research2010
Quadruplex structures of muscle gene promoter sequences enhance in vivo MyoD-dependent gene expression.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Toward an Integrated Strategy for Volumetric Muscle Loss Regeneration.Journal of clinical medicine · 2026Review
- Expression mechanisms of mir-486-5p and its host gene sANK1 in porcine muscle.Molecular biology reports · 2024Article
- Targeting specific DNA G-quadruplexes with CRISPR-guided G-quadruplex-binding proteins and ligands.Nature cell biology · 2024Article
- Roles of G4-DNA and G4-RNA in Class Switch Recombination and Additional Regulations in B-Lymphocytes.Molecules (Basel, Switzerland) · 2023Review
- Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences.Nucleic acids research · 2022Article
- Mechanisms of Binding Specificity among bHLH Transcription Factors.International journal of molecular sciences · 2021Review
- Developing Novel G-Quadruplex Ligands: from Interaction with Nucleic Acids to Interfering with Nucleic Acid⁻Protein Interaction.Molecules (Basel, Switzerland) · 2019Review
- BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori.Nucleic acids research · 2018Article
- Myoblast Myogenic Differentiation but Not Fusion Process Is Inhibited via MyoD Tetraplex Interaction.Oxidative medicine and cellular longevity · 2018Article
- Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken.International journal of molecular sciences · 2016Article
- MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis.International journal of molecular sciences · 2015Article
- TMPyP4, a Stabilizer of Nucleic Acid Secondary Structure, Is a Novel Acetylcholinesterase Inhibitor.PloS one · 2015Article
- Epigenetic modification, dehydration, and molecular crowding effects on the thermodynamics of i-motif structure formation from C-rich DNA.Biochemistry · 2014Article
- Article
- Genome-wide study predicts promoter-G4 DNA motifs regulate selective functions in bacteria: radioresistance of D. radiodurans involves G4 DNA-mediated regulation.Nucleic acids research · 2013Article
- Possible regulatory roles of promoter g-quadruplexes in cardiac function-related genes - human TnIc as a model.PloS one · 2013Article
- DNA secondary structures: stability and function of G-quadruplex structures.Nature reviews. Genetics · 2012Review
- Quadruplex-single nucleotide polymorphisms (Quad-SNP) influence gene expression difference among individuals.Nucleic acids research · 2012Article
- Bisquinolinium compounds induce quadruplex-specific transcriptome changes in HeLa S3 cell lines.BMC research notes · 2012Article
- The evolving world of protein-G-quadruplex recognition: a medicinal chemist's perspective.Biochimie · 2011Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene promoters are enriched in guanine clusters that potentially fold into quadruplex structures. Such quadruplexes were implicated in the regulation of gene expression, plausibly by interacting with transcription factors. We showed previously that homodimers of the myogenic transcription factor MyoD bound in vitro most tightly bimolecular quadruplexes of promoter sequences of muscle-specific genes. By contrast, MyoD-E47 heterodimers formed tighter complexes with d(CANNTG) E-box motifs that govern muscle gene expression. Here, we show that DNA quadruplexes enhance in vivo MyoD and E-box-driven expression of a firefly luciferase (FL) reporter gene. HEK293 cells were transfected with FL expressing p4RTK-FL vector alone or together with MyoD expressing pEMSV-MyoD plasmid, with quadruplexes of alpha7 integrin or sarcomeric mitochondrial creatine kinase (sMtCK) muscle gene promoters or with a combination thereof. Whereas MyoD elevated by approximately 10-fold the levels of FL mRNA and protein, the DNA quadruplexes by themselves did not affect FL expression. However, together with MyoD, quadruplex DNA increased by approximately 35-fold the amounts of FL mRNA and protein. Without affecting its expression, DNA quadruplexes bound MyoD in the cells. Based on these results, we propose models for the regulation of muscle gene transcription by direct interaction of MyoD with promoter quadruplex structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.