ArticleNucleic acids research2010
Yeast arginine methyltransferase Hmt1p regulates transcription elongation and termination by methylating Npl3p.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Expression of spider silk protein in tobacco improves drought tolerance with minimal effects on its mechanotype.The Plant journal : for cell and molecular biology · 2025Article
- Citrullination and the protein code: crosstalk between post-translational modifications in cancer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Review
- RNA recognition by Npl3p reveals U2 snRNA-binding compatible with a chaperone role during splicing.Nature communications · 2023Article
- A Protein-Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20.International journal of molecular sciences · 2023Article
- Protein arginine methylation in transcription and epigenetic regulation.Frontiers in epigenetics and epigenomics · 2023Review
- Post-transcriptional negative feedback regulation of proteostasis through the Dis3 ribonuclease and its disruption by polyQ-expanded Huntingtin.Nucleic acids research · 2019Article
- Experimental evolution reveals a general role for the methyltransferase Hmt1 in noise buffering.PLoS biology · 2019Article
- The RNA binding protein Npl3 promotes resection of DNA double-strand breaks by regulating the levels of Exo1.Nucleic acids research · 2017Article
- Article
- Loss of the Yeast SR Protein Npl3 Alters Gene Expression Due to Transcription Readthrough.PLoS genetics · 2015Article
- Coupling and coordination in gene expression processes with pre-mRNA splicing.International journal of molecular sciences · 2015Review
- Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing.Nucleic acids research · 2014Article
- Disengaging polymerase: terminating RNA polymerase II transcription in budding yeast.Biochimica et biophysica acta · 2013Review
- S-adenosyl-L-homocysteine hydrolase and methylation disorders: yeast as a model system.Biochimica et biophysica acta · 2013Review
- Genomic insights of protein arginine methyltransferase Hmt1 binding reveals novel regulatory functions.BMC genomics · 2012Article
- RGG motif proteins: modulators of mRNA functional states.Cell cycle (Georgetown, Tex.) · 2012Review
- The AMT1 arginine methyltransferase gene is important for plant infection and normal hyphal growth in Fusarium graminearum.PloS one · 2012Article
- Keeping mRNPs in check during assembly and nuclear export.Nature reviews. Molecular cell biology · 2011Article
- The Role of Protein Arginine Methylation in mRNP Dynamics.Molecular biology international · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The heterogeneous nuclear ribonucleoprotein Npl3p of budding yeast is a substrate of arginine methyltransferase Hmt1p, but the role of Hmt1p in regulating Npl3p's functions in transcription antitermination and elongation were unknown. We found that mutants lacking Hmt1p methyltransferase activity exhibit reduced recruitment of Npl3p, but elevated recruitment of a component of mRNA cleavage/termination factor CFI, to the activated GAL10-GAL7 locus. Consistent with this, hmt1 mutants displayed increased termination at the defective gal10-Delta56 terminator. Remarkably, hmt1Delta cells also exhibit diminished recruitment of elongation factor Tho2p and a reduced rate of transcription elongation in vivo. Importantly, the defects in Npl3p and Tho2p recruitment, antitermination and elongation in hmt1Delta cells all were mitigated by substitutions in Npl3p RGG repeats that functionally mimic arginine methylation by Hmt1p. Thus, Hmt1p promotes elongation and suppresses termination at cryptic terminators by methylating RGG repeats in Npl3p. As Hmt1p stimulates dissociation of Tho2p from an Npl3p-mRNP complex, it could act to recycle these elongation and antitermination factors back to sites of ongoing transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.