ArticleMolecular biology of the cell2010
Numb independently antagonizes Sanpodo membrane targeting and Notch signaling in Drosophila sensory organ precursor cells.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Spatio-Temporal Regulation of Notch Activation in Asymmetrically Dividing Sensory Organ Precursor Cells inCells · 2024Review
- Review
- Notch, Numb and Numb-like responses to exercise-induced muscle damage in human skeletal muscle.Experimental physiology · 2022Article
- Endocytic Adaptor Proteins in Health and Disease: Lessons from Model Organisms and Human Mutations.Cells · 2019Review
- Numb regulates the balance between Notch recycling and late-endosome targeting in Drosophila neural progenitor cells.Molecular biology of the cell · 2016Article
- Article
- The association of cholesterol absorption gene Numb polymorphism with Coronary Artery Disease among Han Chinese and Uighur Chinese in Xinjiang, China.Lipids in health and disease · 2015Article
- Genetic variants of numb gene were associated with elevated total cholesterol level and low density lipoprotein cholesterol level in Chinese subjects, in Xinjiang, China.Diagnostic pathology · 2015Article
- A fluorescent tagging approach in Drosophila reveals late endosomal trafficking of Notch and Sanpodo.The Journal of cell biology · 2014Article
- Tools and methods for studying Notch signaling in Drosophila melanogaster.Methods (San Diego, Calif.) · 2014Article
- Adaptor proteins involved in polarized sorting.The Journal of cell biology · 2014Review
- The clathrin adaptor Numb regulates intestinal cholesterol absorption through dynamic interaction with NPC1L1.Nature medicine · 2014Article
- Membrane traffic research: challenges for the next decade.Frontiers in cell and developmental biology · 2014Article
- Cargo recognition in clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2013Review
- Sanpodo controls sensory organ precursor fate by directing Notch trafficking and binding γ-secretase.The Journal of cell biology · 2013Article
- Endocytosis and control of Notch signaling.Current opinion in cell biology · 2012Review
- Interaction of Notch signaling modulator Numb with α-Adaptin regulates endocytosis of Notch pathway components and cell fate determination of neural stem cells.The Journal of biological chemistry · 2012Article
- Endocytosis by Numb breaks Notch symmetry at cytokinesis.Nature cell biology · 2012Article
- dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions.The Journal of cell biology · 2012Article
- The TSC1/2 complex controls Drosophila pigmentation through TORC1-dependent regulation of catecholamine biosynthesis.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In Drosophila, mitotic neural progenitor cells asymmetrically segregate the cell fate determinant Numb in order to block Notch signaling in only one of the two daughter cells. Sanpodo, a membrane protein required for Notch signaling in asymmetrically dividing cells, is sequestered from the plasma membrane to intracellular vesicles in a Numb-dependent way after neural progenitor cell mitosis. However, the significance of Numb-dependent Sanpodo regulation is unclear. In this study, we conducted a structure-function analysis to identify the determinants of Sanpodo targeting in vivo. We identified an NPAF motif in the amino-terminal cytoplasmic tail of Sanpodo, which is conserved among insect Sanpodo homologues. The Sanpodo NPAF motif is predicted to bind directly to the Numb phosphotyrosine-binding domain and is critical for Numb binding in vitro. Deletion or mutation of the NPAF motif results in accumulation of Sanpodo at the plasma membrane in Numb-positive cells in vivo. Genetic analysis of Sanpodo NPAF mutants shows that Numb-dependent Sanpodo endocytic targeting can be uncoupled from Notch signaling regulation. Our findings demonstrate that Sanpodo contains an evolutionarily conserved motif that has been linked to Numb-dependent regulation in vertebrates and further support the model that Numb regulates Notch signaling independently of Sanpodo membrane trafficking in neural progenitor cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.