ArticleJournal of cell science2010
Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.
Article in Journal of cell science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 56 citations in OpenAlex.
- The localisation and stability of the CENP-F protein are regulated by importin beta and microtubules in mitotic cells.Scientific reports · 2025Article
- Functional-proteomics-based investigation of the cellular response to farnesyltransferase inhibition in lung cancer.iScience · 2025Article
- STYK1/NOK affects cell cycle late mitosis and directly interacts with anaphase-promoting complex activator CDH1.Heliyon · 2022Article
- Combined multiomics analysis reveals the mechanism of CENPF overexpression-mediated immune dysfunction in diffuse large B-cell lymphomaFrontiers in genetics · 2022Article
- Screening of Hub Genes in Hepatocellular Carcinoma Based on Network Analysis and Machine Learning.Computational and mathematical methods in medicine · 2022Article
- Discovery and Validation of a Compound to Target Ewing's Sarcoma.Pharmaceutics · 2021Article
- In silico APC/C substrate discovery reveals cell cycle-dependent degradation of UHRF1 and other chromatin regulators.PLoS biology · 2020Article
- Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.eLife · 2020Article
- Review
- Loss of CENPF leads to developmental failure in mouse embryos.Cell cycle (Georgetown, Tex.) · 2019Article
- Miro-dependent mitochondrial pool of CENP-F and its farnesylated C-terminal domain are dispensable for normal development in mice.PLoS genetics · 2019Article
- Disentangling the molecular determinants for Cenp-F localization to nuclear pores and kinetochores.EMBO reports · 2018Article
- Regulation of Cenp-F localization to nuclear pores and kinetochores.Cell cycle (Georgetown, Tex.) · 2018Article
- CENP-F couples cargo to growing and shortening microtubule ends.Molecular biology of the cell · 2017Article
- An integrated bioinformatics platform for investigating the human E3 ubiquitin ligase-substrate interaction network.Nature communications · 2017Article
- Mechanism for G2 phase-specific nuclear export of the kinetochore protein CENP-F.Cell cycle (Georgetown, Tex.) · 2017Article
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- Plk1-mediated stabilization of 53BP1 through USP7 regulates centrosome positioning to maintain bipolarity.Oncogene · 2017Article
- Taming the Beast: Control of APC/CCold Spring Harbor symposia on quantitative biology · 2017Article
- Article
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
Progression through mitosis and cytokinesis requires the sequential proteolysis of several cell-cycle regulators. This proteolysis is mediated by the ubiquitin-proteasome system, with the E3 ligase being the anaphase-promoting complex, also known as the cyclosome (APC/C). The APC/C is regulated by two activators, namely Cdc20 and Cdh1. The current view is that prior to anaphase, the APC/C is activated by Cdc20, but that following anaphase, APC/C switches to Cdh1-dependent activation. However, here we present an analysis of the kinetochore protein Cenp-F that is inconsistent with this notion. Although it has long been appreciated that Cenp-F is degraded sometime during or after mitosis, exactly when and how has not been clear. Here we show that degradation of Cenp-F initiates about six minutes after anaphase, and that this is dependent on a C-terminal KEN-box. Although these two observations are consistent with Cenp-F being a substrate of Cdh1-activated APC/C, Cenp-F is degraded normally in Cdh1-null cells. By contrast, RNAi-mediated repression of APC/C subunits or Cdc20 does inhibit Cenp-F degradation. These findings therefore suggest that the APC/C does not simply 'switch' upon anaphase onset; rather, our observations indicate that Cdc20 also contributes to post-anaphase activation of the APC/C. We also show that the post-anaphase, KEN-box-dependent degradation of Cenp-F requires it to be farnesylated, a post-translational modification usually linked to membrane association. Because so many of the behaviours of Cenp-F are farnesylation-dependent, we suggest that this modification plays a more global role in Cenp-F function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.