ArticleFungal genetics and biology : FG & B2010
Circadian rhythms in Neurospora crassa: dynamics of the clock component frequency visualized using a fluorescent reporter.
Article in Fungal genetics and biology : FG & B, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Trends in circadian rhythms research in fungi since the millenium.Npj biological timing and sleep · 2026Review
- Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia.The Journal of cell biology · 2026Article
- Casein kinase 1a mediates a two-step subunit remodeling mechanism to regulate the FRQ-FRH circadian clock complex.Nature communications · 2026Article
- Core Clock Protein Subcellular Dynamics Coordinate Local and Global Circadian Control in Syncytia.bioRxiv : the preprint server for biology · 2025Article
- Origin of the clock inFrontiers in molecular biosciences · 2025Review
- The clock in growing hyphae and their synchronization in Neurospora crassa.Communications biology · 2024Article
- Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa.Fungal genetics and biology : FG & B · 2023Article
- The macroscopic limit to synchronization of cellular clocks in single cells of Neurospora crassa.Scientific reports · 2022Article
- Identifying a stochastic clock network with light entrainment for single cells of Neurospora crassa.Scientific reports · 2020Article
- What is Phase in Cellular Clocks?The Yale journal of biology and medicine · 2019Article
- Ensemble methods for stochastic networks with special reference to the biological clock of Neurospora crassa.PloS one · 2018Article
- Article
- Synchronizing stochastic circadian oscillators in single cells of Neurospora crassa.Scientific reports · 2016Article
- Multiscale modeling of tumor growth induced by circadian rhythm disruption in epithelial tissue.Journal of biological physics · 2016Article
- Temperature compensation and temperature sensation in the circadian clock.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Identification and characterization of LFD-2, a predicted fringe protein required for membrane integrity during cell fusion in neurospora crassa.Eukaryotic cell · 2015Article
- HAM-5 functions as a MAP kinase scaffold during cell fusion in Neurospora crassa.PLoS genetics · 2014Article
- Efficient detection of unpaired DNA requires a member of the rad54-like family of homologous recombination proteins.Genetics · 2014Article
- High-resolution spatiotemporal analysis of gene expression in real time: in vivo analysis of circadian rhythms in Neurospora crassa using a FREQUENCY-luciferase translational reporter.Fungal genetics and biology : FG & B · 2012Article
- Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica.AMB Express · 2012Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The frequency (frq) gene of Neurospora crassa has long been considered essential to the function of this organism's circadian rhythm. Increasingly, deciphering the coupling of core oscillator genes such as frq to the output pathways of the circadian rhythm has become a major focus of circadian research. To address this coupling it is critical to have a reporter of circadian activity that can deliver high resolution spatial and temporal information about the dynamics of core oscillatory proteins such as FRQ. However, due to the difficulty of studying the expression of circadian rhythm genes in aerobic N. crassa cultures, little is known about the dynamics of this gene under physiologically realistic conditions. To address these issues we report a fluorescent fusion to the frq gene using a codon optimized version of the mCherry gene. To trace the expression and accumulation of FRQ-mCherryNC (FRQ-mCh) during the circadian rhythm, growing vegetative hyphae were scanned every hour under confocal microscopy (100x). Fluorescence of FRQ-mCh was detected only at the growing edge of the colony, and located in the cytoplasm and nuclei of vegetative hyphae for a distance of approximately 150-200microm from the apices of leading hyphae. When driven by the frq promoter, apparently there was also a second FRQ entrance into the nucleus during the circadian cycle; however the second entrance had a lower accumulation level than the first entrance. Thus this fluorescent fusion protein has proven useful in tracking the spatial dynamics of the frq protein and has indicated that the dynamics of the FRQ protein's nuclear trafficking may be more complex than previously realized.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.