ArticleMethods (San Diego, Calif.)2010
Analysis of histones and chromatin in Xenopus laevis egg and oocyte extracts.
Article in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency.iScience · 2024Article
- Independent transcriptomic and proteomic regulation by type I and II protein arginine methyltransferases.iScience · 2021Article
- Real-Time Monitoring of APC /C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Chromatin Characterization inCold Spring Harbor protocols · 2019Article
- Regulation of maintenance DNA methylation via histone ubiquitylation.Journal of biochemistry · 2016Review
- Chaperone-mediated chromatin assembly and transcriptional regulation in Xenopus laevis.The International journal of developmental biology · 2016Review
- Chromatin assembly and transcriptional cross-talk in Xenopus laevis oocyte and egg extracts.The International journal of developmental biology · 2016Article
- Glutamylation of Nap1 modulates histone H1 dynamics and chromosome condensation in Xenopus.The Journal of cell biology · 2015Article
- Developmentally Regulated Post-translational Modification of Nucleoplasmin Controls Histone Sequestration and Deposition.Cell reports · 2015Article
- The intrinsically disordered distal face of nucleoplasmin recognizes distinct oligomerization states of histones.Nucleic acids research · 2014Article
- Phosphorylation and arginine methylation mark histone H2A prior to deposition during Xenopus laevis development.Epigenetics & chromatin · 2014Article
- Looking above but not beyond the genome for therapeutics in neurology and psychiatry: epigenetic proteins and RNAs find a new focus.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2013Article
- Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Histones are the major protein components of chromatin, the physiological form of the genome in all eukaryotic cells. Chromatin is the substrate of information-directed biological processes, such as gene regulation and transcription, replication, and mitosis. A long-standing experimental model system to study many of these processes is the extract made from the eggs of the anuran Xenopus laevis. Since work in recent years has solidified the importance of post-translational modification of histones in directing biological processes, the study of histones in a biochemically dissectible model such as Xenopus is crucial for the understanding of their biological significance. Here we present a rationale and methods for isolating and studying histones and chromatin in different Xenopus egg and oocyte extracts. In particular, we present protocols for the preparation of: cell-free egg and oocyte extract; nucleoplasmic extract ("NPE"); biochemical purification of maternally-deposited, stored histones in the oocyte and the egg; assembly of pronuclei in egg extract and the isolation of pronuclear chromatin and histones; and an extract chromatin assembly assay. We also demonstrate aspects of the variability of the system to be mindful of when working with extract and the importance of proper laboratory temperature in preparing quality extracts. We expect that these methods will be of use in promoting further understanding of embryonic chromatin in a unique experimental system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.