ArticleTraffic (Copenhagen, Denmark)2010
Gangliosides and beta1-integrin are required for caveolae and membrane domains.
Article in Traffic (Copenhagen, Denmark), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- The role of caveolin-1 in atherosclerosis and its molecular mechanism.Lipids in health and disease · 2026Review
- Environmental Toxicants and Their Disruption of Integrin Signaling in Lipid Rafts.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Staphylococcus aureus can use an alternative pathway to be internalized by osteoblasts in absence of β1 integrins.Scientific reports · 2024Article
- The M2 proteins of bat influenza A viruses reveal atypical features compared to conventional M2 proteins.Journal of virology · 2023Article
- Targeted Liposomes Encapsulating miR-603 Complexes Enhance Radiation Sensitivity of Patient-Derived Glioblastoma Stem-Like Cells.Pharmaceutics · 2021Article
- Caveolin1 Tyrosine-14 Phosphorylation: Role in Cellular Responsiveness to Mechanical Cues.The Journal of membrane biology · 2020Review
- Lipid accumulation controls the balance between surface connection and scission of caveolae.eLife · 2020Article
- Article
- Reduced GM1 ganglioside in CFTR-deficient human airway cells results in decreased β1-integrin signaling and delayed wound repair.American journal of physiology. Cell physiology · 2014Article
- Neu3 sialidase-mediated ganglioside conversion is necessary for axon regeneration and is blocked in CNS axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- The epithelial calcium channel TRPV5 is regulated differentially by klotho and sialidase.The Journal of biological chemistry · 2013Article
- Shear Stress Activates eNOS at the Endothelial Apical Surface ThroughCellular and molecular bioengineering · 2013Article
- Overexpression of caveolin-1 is sufficient to phenocopy the behavior of a disease-associated mutant.Traffic (Copenhagen, Denmark) · 2013Article
- Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis.Biochemical and biophysical research communications · 2013Article
- Caveolae as plasma membrane sensors, protectors and organizers.Nature reviews. Molecular cell biology · 2013Review
- The plasma membrane sialidase NEU3 regulates the malignancy of renal carcinoma cells by controlling β1 integrin internalization and recycling.The Journal of biological chemistry · 2012Article
- Coordinated Mechanosensitivity of Membrane Rafts and Focal Adhesions.Cellular and molecular bioengineering · 2012Article
- Oligomers of the ATPase EHD2 confine caveolae to the plasma membrane through association with actin.The EMBO journal · 2012Article
- Switching the targeting pathways of a therapeutic antibody by nanodesign.Angewandte Chemie (International ed. in English) · 2012Article
- Insights on the trafficking and retro-translocation of glycosphingolipid-binding bacterial toxins.Frontiers in cellular and infection microbiology · 2012Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Caveolae are plasma membrane domains involved in the uptake of certain pathogens and toxins. Internalization of some cell surface integrins occurs via caveolae suggesting caveolae may play a crucial role in modulating integrin-mediated adhesion and cell migration. Here we demonstrate a critical role for gangliosides (sialo-glycosphingolipids) in regulating caveolar endocytosis in human skin fibroblasts. Pretreatment of cells with endoglycoceramidase (cleaves glycosphingolipids) or sialidase (modifies cell surface gangliosides and glycoproteins) selectively inhibited caveolar endocytosis by >70%, inhibited the formation of plasma membrane domains enriched in sphingolipids and cholesterol ('lipid rafts'), reduced caveolae and caveolin-1 at the plasma membrane by approximately 80%, and blunted activation of beta1-integrin, a protein required for caveolar endocytosis in these cells. These effects could be reversed by a brief incubation with gangliosides (but not with asialo-gangliosides or other sphingolipids) at 10 degrees C, suggesting that sialo-lipids are critical in supporting caveolar endocytosis. Endoglycoceramidase treatment also caused a redistribution of focal adhesion kinase, paxillin, talin, and PIP Kinase Igamma away from focal adhesions. The effects of sialidase or endoglycoceramidase on membrane domains and the distribution of caveolin-1 could be recapitulated by beta1-integrin knockdown. These results suggest that both gangliosides and beta1-integrin are required for maintenance of caveolae and plasma membrane domains.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.