ArticleJournal of proteome research2010
Quantitative proteomic analysis reveals the perturbation of multiple cellular pathways in HL-60 cells induced by arsenite treatment.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- A Tutorial Review of Labeling Methods in Mass Spectrometry-Based Quantitative Proteomics.ACS measurement science au · 2024Review
- Article
- G3BP1-linked mRNA partitioning supports selective protein synthesis in response to oxidative stress.Nucleic acids research · 2020Article
- SILAC-Based Quantitative Proteomic Analysis Unveils Arsenite-Induced Perturbation of Multiple Pathways in Human Skin Fibroblast Cells.Chemical research in toxicology · 2017Article
- Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells.Scientific reports · 2016Article
- Cd²⁺-induced alteration of the global proteome of human skin fibroblast cells.Journal of proteome research · 2014Article
- Gut microbiome perturbations induced by bacterial infection affect arsenic biotransformation.Chemical research in toxicology · 2013Article
- Quantitative proteomic analysis revealed N'-nitrosonornicotine-induced down-regulation of nonmuscle myosin II and reduced cell migration in cultured human skin fibroblast cells.Journal of proteome research · 2013Article
- Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.International journal of ophthalmology · 2013Article
- Comparative proteomics in acute myeloid leukemia.Contemporary oncology (Poznan, Poland) · 2012Article
- Quantitative proteomic analysis revealed lovastatin-induced perturbation of cellular pathways in HL-60 cells.Journal of proteome research · 2011Article
- Quantification of oxidative DNA lesions in tissues of Long-Evans Cinnamon rats by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope-dilution method.Analytical chemistry · 2011Article
- Quantitative proteomic analysis reveals the perturbation of multiple cellular pathways in jurkat-T cells induced by doxorubicin.Journal of proteome research · 2010Article
- Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.Proteomics · 2010Article
- Clinical proteomics of myeloid leukemia.Genome medicine · 2010Article
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2 authors at 1 institution in 1 country.
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Abstract
Arsenic is ubiquitously present in the environment; it is a known human carcinogen and paradoxically it is also a successful drug for the clinical remission of acute promyelocytic leukemia (APL). The cellular responses induced by arsenite treatment have been investigated for years; however, the precise mechanisms underlying its cytotoxicity and therapeutic activity remain unclear. Here we report the use of mass spectrometry together with stable isotope labeling by amino acids in cell culture (SILAC) for the comparative study of protein expression in HL-60 cells that were untreated or treated with a clinically relevant concentration of arsenite. Our results revealed that, among the 1067 proteins quantified in both forward and reverse SILAC measurements, 56 had significantly altered levels of expression induced by arsenite treatment. These included the up-regulation of core histones, neutrophil elastase, alpha-mannosidase as well as the down-regulation of fatty acid synthase and protein phosphatase 1 alpha. We further demonstrated that the arsenite-induced growth inhibition of HL-60 cells could be rescued by treatment with palmitate, the final product of fatty acid synthase, supporting that arsenite exerts its cytotoxic effect, in part, via suppressing the expression of fatty acid synthase and inhibiting the endogenous production of fatty acid. The results from the present study offered important new knowledge for gaining insights into the molecular mechanisms of action of arsenite.
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