ReviewEcotoxicology (London, England)2010
QPCR: a tool for analysis of mitochondrial and nuclear DNA damage in ecotoxicology.
Review in Ecotoxicology (London, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- Complete Mitochondrial Genome ofGenes · 2026Article
- Defining mutational signatures of lung cancer-associated carcinogens throughbioRxiv : the preprint server for biology · 2026Article
- Alterations in mitochondrial DNA copy number and oxidative stress markers in blood and muscle tissues of mice following snake envenomation.Environmental analysis, health and toxicology · 2026Article
- Utilizing the state of environmental DNA (eDNA) to incorporate time-scale information into eDNA analysis.Proceedings. Biological sciences · 2023Review
- Article
- Neuroligin-mediated neurodevelopmental defects are induced by mitochondrial dysfunction and prevented by lutein in C. elegans.Nature communications · 2022Article
- Current aspects of DNA damage and repair in ecotoxicology: a mini-review.Ecotoxicology (London, England) · 2022Review
- HIV-1 and HIV-1-Tat Induce Mitochondrial DNA Damage in Human Neurons.Journal of HIV and AIDS · 2020Article
- A higher throughput assay for quantification of melphalan-induced DNA damage in peripheral blood mononuclear cells.Scientific reports · 2019Article
- Mitochondria as a target of organophosphate and carbamate pesticides: Revisiting common mechanisms of action with new approach methodologies.Reproductive toxicology (Elmsford, N.Y.) · 2019Article
- Article
- Pathogenic missense mutation pattern of forkhead box genes in neurodevelopmental disorders.Molecular genetics & genomic medicine · 2019Article
- Mitochondrial DNA Damage Does Not DetermineFrontiers in genetics · 2019Article
- Clonal expansion of mitochondrial DNA deletions is a private mechanism of aging in long-lived animals.Aging cell · 2018Article
- Quantitative Extra Long PCR to Detect DNA Lesions in Patients Exposed to Low Doses of Diagnostic RadiationAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Newly Revised Quantitative PCR-Based Assay for Mitochondrial and Nuclear DNA Damage.Current protocols in toxicology · 2018Article
- Clinical effects of chemical exposures on mitochondrial function.Toxicology · 2017Review
- Simple and fast quantification of DNA damage by real-time PCR, and its application to nuclear and mitochondrial DNA from multiple tissues of aging zebrafish.BMC research notes · 2017Article
- Effects of methyl and inorganic mercury exposure on genome homeostasis and mitochondrial function in Caenorhabditis elegans.DNA repair · 2017Article
- Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
The quantitative PCR (QPCR) assay for DNA damage and repair has been used extensively in laboratory species. More recently, it has been adapted to ecological settings. The purpose of this article is to provide a detailed methodological guide that will facilitate its adaptation to additional species, highlight its potential for ecotoxicological and biomonitoring work, and critically review the strengths and limitations of this assay. Major strengths of the assay include very low (nanogram to picogram) amounts of input DNA; direct comparison of damage and repair in the nuclear and mitochondrial genomes, and different parts of the nuclear genome; detection of a wide range of types of DNA damage; very good reproducibility and quantification; applicability to properly preserved frozen samples; simultaneous monitoring of relative mitochondrial genome copy number; and easy adaptation to most species. Potential limitations include the limit of detection (approximately 1 lesion per 10(5) bases); the inability to distinguish different types of DNA damage; and the need to base quantification of damage on a control or reference sample. I suggest that the QPCR assay is particularly powerful for some ecotoxicological studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.