Evidence map›Paper›PMID 20049485›Full record

ArticleJournal of gastroenterology2010

Protein profiles of peripheral blood mononuclear cells are useful for differential diagnosis of ulcerative colitis and Crohn's disease.

Moriaki Hatsugai, Manae S Kurokawa, Takefumi Kouro, Kohei Nagai, Mitsumi Arito, Kayo Masuko, Naoya Suematsu, Kazuki Okamoto, Fumio Itoh, Tomohiro Kato

Registry-linked trialAbstract read
PubMed Publisher
In one paragraph

Article in Journal of gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03689257 (Prospective and Multicenter Study About the Epidemiology and the Characteristics "Omics" in Patients Recently Diagnosed of Inflammatory Bowel Disease in Spain), which is not on this map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03689257 completednot on this mapstarted 2017, after this paper: background citation

Prospective and Multicenter Study About the Epidemiology and the Characteristics "Omics" in Patients Recently Diagnosed of Inflammatory Bowel Disease in Spain

TypeobservationalSponsorFundación de Investigación Biomédica - Hospital Universitario de La PrincesaRan2017 to 2020Enrolled376ConditionsInflammatory Bowel Diseases
3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 48 citations in OpenAlex.

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  14. New Insights into the Role of Trace Elements in IBD.BioMed research international · 2018
    Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Serum protein profiling of adults and children with Crohn disease.Journal of pediatric gastroenterology and nutrition · 2015
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Moriaki HatsugaiClinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Miyamae-ku, Kawasaki, Japan.
Manae S Kurokawa
Takefumi Kouro
Kohei Nagai
Mitsumi Arito
Kayo Masuko
Naoya Suematsu
Kazuki Okamoto
Fumio Itoh
Tomohiro Kato
St. Marianna University School of Medicine · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEffective biomarkers for discrimination between ulcerative colitis (UC) and Crohn's disease (CD) have not been established yet. In this study, we analyzed protein profiles of peripheral blood mononuclear cells (PBMCs) of the patients to find such a biomarker.

methodsPeripheral blood mononuclear cell proteins from 17 UC patients, 13 CD patients, and 17 healthy controls were separated by two-dimensional gel electrophoresis. The intensities of individual protein spots were subjected to discriminant analysis of UC and CD using the SIMCA-P+program.

resultsWe found that 547 protein spots were commonly detected among the UC, CD, and healthy groups. Orthogonal partial least squares-discriminant analysis using 276 protein spots clearly discriminated the UC patients from the CD patients (R (2) 0.994; Q (2) 0.462). A similar analysis using a further selected 58 protein spots showed higher performance for discrimination of the diseases (R (2) 0.948; Q (2) 0.566). Eleven out of the 58 protein spots were successfully identified; these were functionally related to inflammation, oxidation/reduction, the cytoskeleton, endocytotic trafficking, and transcription. In addition, the PBMC protein profiles were useful for the prediction of disease activity in the UC and the CD patients, and they were also useful for predicting disease severity and responses to treatments in the UC patients.

conclusionsPBMC protein profiles are useful for the discrimination of UC from CD. The profiles could be a potent biomarker for the differential diagnosis of these diseases. Further investigation of the proteins which contributed to the discrimination could promote elucidation of the pathophysiology of UC and CD.

Indexed as

AdolescentAdultAgedBiomarkersBlood ProteinsCase-Control StudiesColitis, UlcerativeCrohn DiseaseDiagnosis, DifferentialDiscriminant AnalysisFemaleHumansLeast-Squares AnalysisLeukocytes, MononuclearMaleMiddle AgedBiomarkersBlood Proteins

Identifiers

PMID20049485
OpenAlexW2148513671

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.