ArticleThe Journal of biological chemistry2010
Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 61 citations in OpenAlex.
- Building a digital library on research into mineralizing vesicles: a systematic review-based approach.Biophysical reviews · 2025Review
- Review
- NPP1 and TNAP hydrolyze ATP synergistically during biomineralization.Purinergic signalling · 2023Article
- Weighing the Evidence for the Roles of Plasma Versus Local Pyrophosphate in Ectopic Calcification Disorders.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- The Application of Black Phosphorus Nanomaterials in Bone Tissue Engineering.Pharmaceutics · 2022Review
- Langmuir monolayers and proteoliposomes as models of matrix vesicles involved in biomineralization.Biophysical reviews · 2021Article
- Matrix Vesicles: Role in Bone Mineralization and Potential Use as Therapeutics.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Phosphatidylserine controls calcium phosphate nucleation and growth on lipid monolayers: A physicochemical understanding of matrix vesicle-driven biomineralization.Journal of structural biology · 2020Article
- Lipid composition modulates ATP hydrolysis and calcium phosphate mineral propagation by TNAP-harboring proteoliposomes.Archives of biochemistry and biophysics · 2020Article
- Matrix vesicle biomimetics harboring Annexin A5 and alkaline phosphatase bind to the native collagen matrix produced by mineralizing vascular smooth muscle cells.Biochimica et biophysica acta. General subjects · 2020Article
- Cholesterol Regulates the Incorporation and Catalytic Activity of Tissue-Nonspecific Alkaline Phosphatase in DPPC Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators.Journal of bone and mineral metabolism · 2019Article
- Bioinspired extracellular vesicles embedded with black phosphorus for molecular recognition-guided biomineralization.Nature communications · 2019Article
- Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?Archives of biochemistry and biophysics · 2019Article
- Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models.Biochimica et biophysica acta. General subjects · 2018Review
- Biophysical aspects of biomineralization.Biophysical reviews · 2017Review
- Biomedical applications of nanotechnology.Biophysical reviews · 2017Review
- Pendant-drop method coupled to ultraviolet-visible spectroscopy: A useful tool to investigate interfacial phenomena.Colloids and surfaces. A, Physicochemical and engineering aspects · 2016Article
- The two sides of a lipid-protein story.Biophysical reviews · 2016Review
- Skeletal Mineralization Deficits and Impaired Biogenesis and Function of Chondrocyte-Derived Matrix Vesicles in Phospho1(-/-) and Phospho1/Pi t1 Double-Knockout Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
We have established a proteoliposome system as an osteoblast-derived matrix vesicle (MV) biomimetic to facilitate the study of the interplay of tissue-nonspecific alkaline phosphatase (TNAP) and NPP1 (nucleotide pyrophosphatase/phosphodiesterase-1) during catalysis of biomineralization substrates. First, we studied the incorporation of TNAP into liposomes of various lipid compositions (i.e. in pure dipalmitoyl phosphatidylcholine (DPPC), DPPC/dipalmitoyl phosphatidylserine (9:1 and 8:2), and DPPC/dioctadecyl-dimethylammonium bromide (9:1 and 8:2) mixtures. TNAP reconstitution proved virtually complete in DPPC liposomes. Next, proteoliposomes containing either recombinant TNAP, recombinant NPP1, or both together were reconstituted in DPPC, and the hydrolysis of ATP, ADP, AMP, pyridoxal-5'-phosphate (PLP), p-nitrophenyl phosphate, p-nitrophenylthymidine 5'-monophosphate, and PP(i) by these proteoliposomes was studied at physiological pH. p-Nitrophenylthymidine 5'-monophosphate and PLP were exclusively hydrolyzed by NPP1-containing and TNAP-containing proteoliposomes, respectively. In contrast, ATP, ADP, AMP, PLP, p-nitrophenyl phosphate, and PP(i) were hydrolyzed by TNAP-, NPP1-, and TNAP plus NPP1-containing proteoliposomes. NPP1 plus TNAP additively hydrolyzed ATP, but TNAP appeared more active in AMP formation than NPP1. Hydrolysis of PP(i) by TNAP-, and TNAP plus NPP1-containing proteoliposomes occurred with catalytic efficiencies and mild cooperativity, effects comparable with those manifested by murine osteoblast-derived MVs. The reconstitution of TNAP and NPP1 into proteoliposome membranes generates a phospholipid microenvironment that allows the kinetic study of phosphosubstrate catabolism in a manner that recapitulates the native MV microenvironment.
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