ArticlePhilosophical transactions. Series A, Mathematical, physical, and engineering sciences2010
Fixed negative charge and the Donnan effect: a description of the driving forces associated with brain tissue swelling and oedema.
Article in Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 52 citations in OpenAlex.
- A mechanovascular framework for pre-neoplastic microenvironmental dysregulation and early carcinogenesis.Frontiers in oncology · 2026Article
- Experimental Measurement and Mathematical Quantification of Fixed-Charged Density in Rat and Pig Brain Tissue.Annals of biomedical engineering · 2025Article
- Separation effectiveness of ideal ion exchange membranes: Application of the Gibbs-Donnan theory.PloS one · 2025Article
- A rotenone organotypic whole hemisphere slice model of mitochondrial abnormalities in the neonatal brain.Journal of biological engineering · 2024Article
- Alterations in brain fluid physiology during the early stages of development of ischaemic oedema.Fluids and barriers of the CNS · 2024Review
- Acute brain slice elastic modulus decreases over time.Scientific reports · 2023Article
- Comparative Study of Terminal Cortical Potentials Using Iridium and Ag/AgCl Electrodes.International journal of molecular sciences · 2023Article
- A Hybrid Reactive Multiphasic Mixture With a Compressible Fluid Solvent.Journal of biomechanical engineering · 2022Article
- Calculation of the Gibbs-Donnan factors for multi-ion solutions with non-permeating charge on both sides of a permselective membrane.Scientific reports · 2021Article
- Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.International journal of nanomedicine · 2021Article
- Reappraisal of anoxic spreading depolarization as a terminal event during oxygen-glucose deprivation in brain slices in vitro.Scientific reports · 2020Article
- Hyaluronidase reduced edema after experimental traumatic brain injury.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Article
- Softening of the chronic hemi-section spinal cord injury scar parallels dysregulation of cellular and extracellular matrix content.Journal of the mechanical behavior of biomedical materials · 2020Article
- Inward Tension of Talin and Integrin-related Osmotic Pressure are involved Synergetically in the Invasion and Metastasis of Non-small Cell Lung Cancer.Journal of Cancer · 2020Article
- Influences of sodium and glycosaminoglycans on skin oedema and the potential for ulceration: a finite-element approach.Royal Society open science · 2019Article
- Biomechanical simulation of traumatic brain injury in the rat.Clinical biomechanics (Bristol, Avon) · 2019Review
- Intracellular ion and protein nanoparticle-induced osmotic pressure modify astrocyte swelling and brain edema in response to glutamate stimuli.Redox biology · 2019Article
- Dynamics of the Hypoxia-Induced Tissue Edema in the Rat Barrel CortexFrontiers in cellular neuroscience · 2018Article
- Intracerebroventricular administration of chondroitinase ABC reduces acute edema after traumatic brain injury in mice.BMC research notes · 2016Article
- Mechanics of the brain: perspectives, challenges, and opportunities.Biomechanics and modeling in mechanobiology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cerebral oedema or brain tissue swelling is a significant complication following traumatic brain injury or stroke that can increase the intracranial pressure (ICP) and impair blood flow. Here, we have identified a potential driver of oedema: the negatively charged molecules fixed within cells. This fixed charge density (FCD), once exposed, could increase ICP through the Donnan effect. We have shown that metabolic processes and membrane integrity are required for concealing this FCD as slices of rat cortex swelled immediately (within 30 min) following dissection if treated with 2 deoxyglucose + cyanide (2DG+CN) or Triton X-100. Slices given ample oxygen and glucose, however, did not swell significantly. We also found that dead brain tissue swells and shrinks in response to changes in ionic strength of the bathing medium, which suggests that the Donnan effect is capable of pressurizing and swelling brain tissue. As predicted, a non-ionic osmolyte, 1,2 propanediol, elicited no volume change at 2000 x 10(-3) osmoles l(-1) (Osm). Swelling data were well described by triphasic mixture theory with the calculated reference state FCD similar to that measured with a 1,9 dimethylmethylene blue assay. Taken together, these data suggest that intracellular fixed charges may contribute to the driving forces responsible for brain swelling.
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