ArticlePlant molecular biology2010
In vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana.
Article in Plant molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Co-Suppression ofPlants (Basel, Switzerland) · 2020Article
- The Maize Sulfite Reductase Is Involved in Cold and Oxidative Stress Responses.Frontiers in plant science · 2018Article
- Impairment of Sulfite Reductase Decreases Oxidative Stress Tolerance inFrontiers in plant science · 2016Article
- Physiological Functions of the COPI Complex in Higher Plants.Molecules and cells · 2015Article
- Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants.Plant physiology · 2014Article
- DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants.Journal of experimental botany · 2014Article
- Dynamic composition, shaping and organization of plastid nucleoids.Frontiers in plant science · 2014Review
- Review
- Sulfite reductase protects plants against sulfite toxicity.Plant physiology · 2013Article
- Characterization of cell death induced by NbBPS1 silencing in Nicotiana benthamiana.Molecules and cells · 2012Article
- Nucleoid-enriched proteomes in developing plastids and chloroplasts from maize leaves: a new conceptual framework for nucleoid functions.Plant physiology · 2012Article
- PRBP plays a role in plastid ribosomal RNA maturation and chloroplast biogenesis in Nicotiana benthamiana.Planta · 2011Article
- Etioplast and etio-chloroplast formation under natural conditions: the dark side of chlorophyll biosynthesis in angiosperms.Photosynthesis research · 2010Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sulfite reductase (SiR) performs dual functions, acting as a sulfur assimilation enzyme and as a chloroplast (cp-) nucleoid binding protein. In this study, we examined the in vivo effects of SiR deficiency on chloroplast development in Nicotiana benthamiana. Virus-induced gene silencing of NbSiR resulted in leaf yellowing and growth retardation phenotypes, which were not rescued by cysteine supplementation. NbSiR:GFP fusion protein was targeted to chloroplasts and colocalized with cp-nucleoids. Recombinant full-length NbSiR protein and the C-terminal half of NbSiR possessed cp-DNA compaction activities in vitro, and expression of full-length NbSiR in E. coli caused condensation of genomic DNA. NbSiR silencing differentially affected expression of plastid-encoded genes, inhibiting expression of several genes more severely than others. In the later stages, depletion of NbSiR resulted in chloroplast ablation. In NbSiR-silenced plants, enlarged cp-nucleoids containing an increased amount of cp-DNA were observed in the middle of the abnormal chloroplasts, and the cp-DNAs were predominantly of subgenomic sizes based on pulse field gel electrophoresis. The abnormal chloroplasts developed prolamellar body-like cubic lipid structures in the light without accumulating NADPH:protochlorophyllide oxidoreductase proteins. Our results suggest that NbSiR plays a role in cp-nucleoid metabolism, plastid gene expression, and thylakoid membrane development.
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