ArticleCellular and molecular bioengineering2009
Dynamics of Microvillus Extension and Tether Formation in Rolling Leukocytes.
Article in Cellular and molecular bioengineering, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Nanoscopic Characterization of Cell Migration under Flow Using Optical and Electron Microscopy.Analytical chemistry · 2023Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Elongated neutrophil-derived structures are blood-borne microparticles formed by rolling neutrophils during sepsis.The Journal of experimental medicine · 2021Article
- Rolling neutrophils form tethers and slings under physiologic conditions in vivo.Journal of leukocyte biology · 2018Article
- Microfluidics-based side view flow chamber reveals tether-to-sling transition in rolling neutrophils.Scientific reports · 2016Article
- Role of the endothelial surface layer in neutrophil recruitment.Journal of leukocyte biology · 2015Review
- Article
- Ultrastructural analysis of nanogold-labeled cell surface microvilli in liquid by atmospheric scanning electron microscopy and their relevance in cell adhesion.International journal of molecular sciences · 2013Article
- Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes.EMBO reports · 2013Article
- Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings.Molecular immunology · 2013Review
- Leukocyte rolling on P-selectin: a three-dimensional numerical study of the effect of cytoplasmic viscosity.Biophysical journal · 2012Article
- Ezrin-radixin-moesin-binding sequence of PSGL-1 glycoprotein regulates leukocyte rolling on selectins and activation of extracellular signal-regulated kinases.The Journal of biological chemistry · 2012Article
- Live cell imaging of paxillin in rolling neutrophils by dual-color quantitative dynamic footprinting.Microcirculation (New York, N.Y. : 1994) · 2011Article
- Cell protrusions and tethers: a unified approach.Biophysical journal · 2011Article
- Biomechanics of leukocyte rolling.Biorheology · 2011Review
- Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling.Nature methods · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
P-selectin glycoprotein ligand-1 (PSGL-1) binding to P-selectin mediates leukocyte rolling under conditions of flow. In human neutrophils, a type of leukocyte belonging to the innate immune system, PSGL-1 molecules are located on the neutrophil's surface ruffles, called microvilli. Each newly formed P-selectin-PSGL-1 bond can become load bearing, imposing on its microvillus a pulling force that deforms the microvillus. Depending on the magnitude of the bond force, a microvillus can be extended, or a thin membrane cylinder (a tether) can be formed at the tip of the microvillus. Here we propose a Kelvin-Voigt viscoelastic material as an improved model for microvillus extension. Using a modified version of our Event-Tracking Model of Adhesion (ETMA), we demonstrate how P-selectin-PSGL-1 load-bearing bonds shape microvillus deformation during neutrophil rolling at low shear (wall shear rate of 50 s(-1), P-selectin site density of 150 molecules μm(-2)). We also discuss the impact of microvillus deformability on neutrophil rolling. We find that the average microvillus extension constitutes 65% of the total microvillus-tether complex extension, and that the rolling neutrophil may never fully rest. A quantitative comparison with the corresponding non-deformable microvilli case supports a concept that the ability of the microvillus to deform stabilizes cell rolling.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.