ReviewMethods (San Diego, Calif.)2010
The use of antisense oligonucleotides in Xenopus oocytes.
Review in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- A maternal dorsoventral prepattern revealed by an asymmetric distribution of ventralizing molecules before fertilization inFrontiers in cell and developmental biology · 2024Article
- Lysosomal degradation of the maternal dorsal determinant Hwa safeguards dorsal body axis formation.EMBO reports · 2021Article
- Oocyte Host-Transfer and Maternal mRNA Depletion Experiments inCold Spring Harbor protocols · 2018Article
- Role of maternal Xenopus syntabulin in germ plasm aggregation and primordial germ cell specification.Developmental biology · 2017Article
- Controlling the Messenger: Regulated Translation of Maternal mRNAs in Xenopus laevis Development.Advances in experimental medicine and biology · 2017Review
- Manipulation and in vitro maturation of Xenopus laevis oocytes, followed by intracytoplasmic sperm injection, to study embryonic development.Journal of visualized experiments : JoVE · 2015Article
- The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos.PloS one · 2015Article
- Differential role of STIM1 and STIM2 during transient inward (T in) current generation and the maturation process in the Xenopus oocyte.BMC physiology · 2014Article
- Nuclear Wave1 is required for reprogramming transcription in oocytes and for normal development.Science (New York, N.Y.) · 2013Article
- Protein interactions in Xenopus germ plasm RNP particles.PloS one · 2013Article
- Maternal mRNA knock-down studies: antisense experiments using the host-transfer technique in Xenopus laevis and Xenopus tropicalis.Methods in molecular biology (Clifton, N.J.) · 2012Article
- RNAi in Xenopus: look before you leap.Genes & development · 2011Article
- Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis.Genes & development · 2011Article
- Use of fully modified 2'-O-methyl antisense oligos for loss-of-function studies in vertebrate embryos.Genesis (New York, N.Y. : 2000) · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability to manipulate gene expression in Xenopus oocytes and then generate fertilized embryos by transfer into host females has made it possible to rapidly characterize maternal signaling pathways in vertebrate development. Maternal mRNAs in particular can be efficiently depleted using antisense deoxyoligonucleotides (oligos), mediated by endogenous RNase-H activity. Since the microinjection of antisense reagents or mRNAs into eggs after fertilization often fails to affect maternal signaling pathways, mRNA depletion in the Xenopus oocyte is uniquely suited to assessing maternal functions. In this review, we highlight the advantages of using antisense in Xenopus oocytes and describe basic methods for designing and choosing effective oligos. We also summarize the procedures for fertilizing cultured oocytes by host-transfer and interpreting the specificity of antisense effects. Although these methods can be technically demanding, the use of antisense in oocytes can be used to address biological questions that are intractable in other experimental settings.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.