ArticleMolecular and biochemical parasitology2010
A dispensable Plasmodium locus for stable transgene expression.
Article in Molecular and biochemical parasitology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.PloS one · 2021Article
- A male gametocyte osmiophilic body and microgamete surface protein of the rodent malaria parasite Plasmodium yoelii (PyMiGS) plays a critical role in male osmiophilic body formation and exflagellation.Cellular microbiology · 2018Article
- The MicronemalFrontiers in cellular and infection microbiology · 2018Article
- Assessment of the Plasmodium falciparum Preerythrocytic Antigen UIS3 as a Potential Candidate for a Malaria Vaccine.Infection and immunity · 2017Article
- Towards genome-wide experimental genetics in the in vivo malaria model parasite Plasmodium berghei.Pathogens and global health · 2015Review
- Imaging Plasmodium immunobiology in the liver, brain, and lung.Parasitology international · 2014Review
- Article
- Quantitative bioluminescent imaging of pre-erythrocytic malaria parasite infection using luciferase-expressing Plasmodium yoelii.PloS one · 2013Article
- Plasmodium yoelii macrophage migration inhibitory factor is necessary for efficient liver-stage development.Infection and immunity · 2012Article
- Neuroimmunological blood brain barrier opening in experimental cerebral malaria.PLoS pathogens · 2012Article
- Salivary gland-specific P. berghei reporter lines enable rapid evaluation of tissue-specific sporozoite loads in mosquitoes.PloS one · 2012Article
- The primase domain of PfPrex is a proteolytically matured, essential enzyme of the apicoplast.Molecular and biochemical parasitology · 2011Article
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5 authors.
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Abstract
The ribosomal small subunit locus has been used for transgene expression in the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, but this strategy utilizes single crossover integration and is thus prone to reversion by plasmid excision. Targeting of the ribosomal subunit locus may also have a negative effect on oocyst development in the mosquito. In P. berghei, the p230 paralog locus has been used for transgene expression. Here, we show that the P. yoelii S1 locus (sporozoite expressed gene 1) (PY05712) is dispensable and can be used for stable transgene expression throughout the parasite life cycle. P. yoelii s1(-) parasites show no defect in blood stage replication, oocyst formation, sporozoite production, or liver stage development when compared to P. yoelii wildtype parasites. Further, we show that a fluorescent transgene can be stably expressed from this site. This demonstrates that the S1 locus can be utilized for stable expression of heterologous genes in rodent malaria parasites.
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