ArticleJournal of Zhejiang University. Science. B2010
A food-grade industrial arming yeast expressing beta-1,3-1,4-glucanase with enhanced thermal stability.
Article in Journal of Zhejiang University. Science. B, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Sugar Utilization-Associated Food-Grade Selection Markers in Lactic Acid Bacteria and Yeast.Polish journal of microbiology · 2024Review
- Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09.Journal of Zhejiang University. Science. B · 2014Article
- Efficient expression of a Paenibacillus barcinonensis endoglucanase in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2012Article
- Production of conjugated linoleic acids by Lactobacillus plantarum strains isolated from naturally fermented Chinese pickles.Journal of Zhejiang University. Science. B · 2011Article
- Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity.Journal of Zhejiang University. Science. B · 2011Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this work was to construct a novel food-grade industrial arming yeast displaying beta-1,3-1,4-glucanase and to evaluate the thermal stability of the glucanase for practical application. For this purpose, a bi-directional vector containing galactokinase (GAL1) and phosphoglycerate kinase 1 (PGK1) promoters in different orientations was constructed. The beta-1,3-1,4-glucanase gene from Bacillus subtilis was fused to alpha-agglutinin and expressed under the control of the GAL1 promoter. alpha-galactosidase induced by the constitutive PGK1 promoter was used as a food-grade selection marker. The feasibility of the alpha-galactosidase marker was confirmed by the growth of transformants harboring the constructed vector on a medium containing melibiose as a sole carbon source, and by the clear halo around the transformants in Congo-red plates owing to the expression of beta-1,3-1,4-glucanase. The analysis of beta-1,3-1,4-glucanase activity in cell pellets and in the supernatant of the recombinant yeast strain revealed that beta-1,3-1,4-glucanase was successfully displayed on the cell surface of the yeast. The displayed beta-1,3-1,4-glucanase activity in the recombinant yeast cells increased immediately after the addition of galactose and reached 45.1 U/ml after 32-h induction. The thermal stability of beta-1,3-1,4-glucanase displayed in the recombinant yeast cells was enhanced compared with the free enzyme. These results suggest that the constructed food-grade yeast has the potential to improve the brewing properties of beer.
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