ArticleAmerican journal of respiratory cell and molecular biology2010
Reduced viability of mice with lung epithelial-specific knockout of glucocorticoid receptor.
Article in American journal of respiratory cell and molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Evolutionary Integration and Glucocorticoid Regulation of the Respiratory System: Structure, Function, and Homeostatic Adaptation.Medical sciences (Basel, Switzerland) · 2026Review
- Embigin deficiency leads to delayed embryonic lung development and high neonatal mortality in mice.iScience · 2024Article
- Impact of downstream effects of glucocorticoid receptor dysfunction on organ function in critical illness-associated systemic inflammation.Intensive care medicine experimental · 2020Review
- Targeted Disruption of Mouse Dip2B Leads to Abnormal Lung Development and Prenatal Lethality.International journal of molecular sciences · 2020Article
- Understanding Human Lung Development through In Vitro Model Systems.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Review
- Autophagy is required for lung development and morphogenesis.The Journal of clinical investigation · 2019Article
- Circadian variation in pulmonary inflammatory responses is independent of rhythmic glucocorticoid signaling in airway epithelial cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Glucocorticoid Signaling in Health and Disease: Insights From Tissue-Specific GR Knockout Mice.Endocrinology · 2018Review
- Article
- Loss of the podocyte glucocorticoid receptor exacerbates proteinuria after injury.Scientific reports · 2017Article
- Epithelial Sodium Channels in Pulmonary Epithelial Progenitor and Stem Cells.International journal of biological sciences · 2016Review
- Maternal high-fat diet is associated with impaired fetal lung development.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Glucocorticoid regulation of lung development: lessons learned from conditional GR knockout mice.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Article
- Two nested developmental waves demarcate a compartment boundary in the mouse lung.Nature communications · 2014Article
- Lung epithelial branching program antagonizes alveolar differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Ceramides: a potential therapeutic target in pulmonary emphysema.Respiratory research · 2013Article
- Glucocorticoid receptor signaling in health and disease.Trends in pharmacological sciences · 2013Review
- Endothelial glucocorticoid receptor is required for protection against sepsis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Deletion of mesenchymal glucocorticoid receptor attenuates embryonic lung development and abdominal wall closure.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Glucocorticoid (GC)-responsive epithelial-mesenchymal interactions regulate lung development. The GC receptor (GR) mediates GC signaling. Mice lacking GR in all tissues die at birth of respiratory failure. To determine the specific need for epithelial GR in lung development, we bred triple transgenic mice that carry SPC/rtTA, tet-O-Cre, and floxed, but not wild-type, GR genes. When exposed to doxycycline in utero, triple transgenic (GRepi⁻) mice exhibit a Cre-mediated recombination event that inactivates the floxed GR gene in airway epithelial cells. Immunofluorescence confirmed the elimination of GR in Cre-positive airway epithelial cells of late gestation GRepi⁻ mice. Embryonic Day 18.5 pups had a relatively immature appearance with increased lung cellularity and increased pools of glycogen in the epithelium. Postnatal Day 0.5 pups had decreased viability. We used quantitative RT-PCR to demonstrate that specific elimination of epithelial immunoreactive GR in GRepi⁻ mice is associated with reduced mRNA expression for surfactant proteins (SPs) A, B, C, and D; β- and γ-ENaC; T1α; the 10-kD Clara cell protein (CCSP); and aquaporin 5 (AQP5). Western blots confirmed reduced levels of AQP5 protein. No reduction in the levels of the GR transport protein importin (IPO)-13 was observed. Our findings demonstrate a requirement for lung epithelial cell GR in normal lung development. We speculate that impaired epithelial differentiation, leading to decreased SPs, transepithelial Na, and liquid absorption at birth, may contribute to the reduced survival of newborn mice with suppressed lung epithelial GR.
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Registered trials
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