ArticleJournal of clinical microbiology2010
Proof of principle for successful characterization of methicillin-resistant coagulase-negative staphylococci isolated from skin by use of Raman spectroscopy and pulsed-field gel electrophoresis.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms.Molecules (Basel, Switzerland) · 2024Review
- Molecular Detection and Characterization of theBioMed research international · 2023Article
- Comparison of Different Label-Free Raman Spectroscopy Approaches for the Discrimination of Clinical MRSA and MSSA Isolates.Microbiology spectrum · 2022Article
- Molecular epidemiology of antimicrobial resistant microorganisms in the 21th century: a review of the literature.Acta bio-medica : Atenei Parmensis · 2020Review
- Clindamycin-rifampin combination therapy for staphylococcal periprosthetic joint infections: a retrospective observational study.BMC infectious diseases · 2017Observational
- Antimicrobial susceptibility testing of Gram-positive and -negative bacterial isolates directly from spiked blood culture media with Raman spectroscopy.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- The Application of Pulsed Field Gel Electrophoresis in Clinical Studies.Journal of clinical and diagnostic research : JCDR · 2016Review
- Raman spectroscopy in biomedicine - non-invasive in vitro analysis of cells and extracellular matrix components in tissues.Biotechnology journal · 2013Review
- Detecting and tracking nosocomial methicillin-resistant Staphylococcus aureus using a microfluidic SERS biosensor.Analytical chemistry · 2013Article
- Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy.Applied and environmental microbiology · 2013Article
- Rapid clinical bacteriology and its future impact.Annals of laboratory medicine · 2013Review
- Comparison of SpectraCell RA typing and multilocus sequence typing for extended-spectrum-β-lactamase-producing Escherichia coli.Journal of clinical microbiology · 2012Article
- Raman spectroscopic analysis of the clonal and horizontal spread of CTX-M-15-producing Klebsiella pneumoniae in a neonatal intensive care unit.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Rapid typing of extended-spectrum β-lactamase- and carbapenemase-producing Escherichia coli and Klebsiella pneumoniae isolates by use of SpectraCell RA.Journal of clinical microbiology · 2012Article
- Coagulase-negative staphylococci: update on the molecular epidemiology and clinical presentation, with a focus on Staphylococcus epidermidis and Staphylococcus saprophyticus.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Review
- Incidence and risk factors of the secondary skin infections in patients with radiodermatitis.The Eurasian journal of medicine · 2011Article
- Raman Spectroscopy: Incorporating the Chemical Dimension into Dermatological Diagnosis.Indian journal of dermatologyArticle
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coagulase-negative staphylococci (CNS) are among the most frequently isolated bacterial species in clinical microbiology, and most CNS-related infections are hospital acquired. Distinguishing between these frequently multiple-antibiotic-resistant isolates is important for both treatment and transmission control. In this study we used isolates of methicillin-resistant coagulase-negative staphylococci (MR-CNS) that were selected from a large surveillance study of the direct spread of MR-CNS. This strain collection was used to evaluate (i) Raman spectroscopy as a typing tool for MR-CNS isolates and (ii) diversity between colonies with identical and different morphologies. Reproducibility was high, with 215 of 216 (99.5%) of the replicate samples for 72 isolates ending up in the same cluster. The concordance with pulsed-field gel electrophoresis (PFGE)-based clusters was 94.4%. We also confirm that the skin of patients can be colonized with multiple MR-CNS types at the same time. Morphological differences between colonies from a single patient sample correlated with differences in Raman and PFGE types. Some morphologically indistinguishable colonies revealed different Raman and PFGE types. This indicates that multiple MR-CNS colonies should be examined to obtain a complete insight into the prevalence of different types and to be able to perform an accurate transmission analysis. Here we show that Raman spectroscopy is a reproducible typing system for MR-CNS isolates. It is a tool for screening variability within a collection of isolates. Because of the high throughput, it enables the analysis of multiple colonies per patient, which will enhance the quality of clinical and epidemiological studies.
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