ArticleBMC developmental biology2009
A laser pointer driven microheater for precise local heating and conditional gene regulation in vivo. Microheater driven gene regulation in zebrafish.
Article in BMC developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Red Blood Cells as Endogenous Biotweezers for Optical Micromanipulation In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Caudal fin shape imprinted during late zebrafish embryogenesis is re-patterned by the Sonic hedgehog pathway.PLoS biology · 2025Article
- An Hsp70 promoter-based mouse for heat shock-induced gene modulation.Journal of molecular medicine (Berlin, Germany) · 2024Article
- Melanocortin Receptor 4 Signaling Regulates Vertebrate Limb Regeneration.Developmental cell · 2018Article
- NIR laser pointer for in vivo photothermal therapy of murine LM3 tumor using intratumoral China ink as a photothermal agent.Lasers in medical science · 2018Article
- Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Temporally coordinated signals progressively pattern the anteroposterior and dorsoventral body axes.Seminars in cell & developmental biology · 2015Review
- Embryonic exposure to excess thyroid hormone causes thyrotrope cell death.The Journal of clinical investigation · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundTissue heating has been employed to study a variety of biological processes, including the study of genes that control embryonic development. Conditional regulation of gene expression is a particularly powerful approach for understanding gene function. One popular method for mis-expressing a gene of interest employs heat-inducible heat shock protein (hsp) promoters. Global heat shock of hsp-promoter-containing transgenic animals induces gene expression throughout all tissues, but does not allow for spatial control. Local heating allows for spatial control of hsp-promoter-driven transgenes, but methods for local heating are cumbersome and variably effective.
resultsWe describe a simple, highly controllable, and versatile apparatus for heating biological tissue and other materials on the micron-scale. This microheater employs micron-scale fiber optics and uses an inexpensive laser-pointer as a power source. Optical fibers can be pulled on a standard electrode puller to produce tips of varying sizes that can then be used to reliably heat 20-100 mum targets. We demonstrate precise spatiotemporal control of hsp70l:GFP transgene expression in a variety of tissue types in zebrafish embryos and larvae. We also show how this system can be employed as part of a new method for lineage tracing that would greatly facilitate the study of organogenesis and tissue regulation at any time in the life cycle.
conclusionThis versatile and simple local heater has broad utility for the study of gene function and for lineage tracing. This system could be used to control hsp-driven gene expression in any organism simply by bringing the fiber optic tip in contact with the tissue of interest. Beyond these uses for the study of gene function, this device has wide-ranging utility in materials science and could easily be adapted for therapeutic purposes in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.