ArticleBMC genomics2009
MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 108 citations in OpenAlex.
- Heat stress-induced remodelling of lipid and microRNA networks in Brassica napus germinated pollen and pollen-derived small extracellular vesicles.Journal of experimental botany · 2026Article
- Suppression of microRNAs targeting key MEP pathway genes affects plastid terpenoid content and photosynthesis under variable light conditions.Molecular biology reports · 2026Article
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- bra-miR9569 Targets thePlants (Basel, Switzerland) · 2025Article
- The miRNome function transitions from regulating developmental genes to transposable elements during pollen maturation.The Plant cell · 2022Article
- MicroRNAs Involved in Regulatory Cytoplasmic Male Sterility by Analysis RNA-seq and Small RNA-seq in Soybean.Frontiers in genetics · 2021Article
- Integrated Proteo-Transcriptomic Analyses Reveal Insights into Regulation of Pollen Development Stages and Dynamics of Cellular Response to Apple Fruit Crinkle Viroid (AFCVd)-Infection inInternational journal of molecular sciences · 2020Article
- Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitro.Protoplasma · 2020Article
- Article
- ARGONAUTE1 and ARGONAUTE4 Regulate Gene Expression and Hypoxia Tolerance.Plant physiology · 2020Article
- Article
- Plasmogamic Paternal Contributions to Early Zygotic Development in Flowering Plants.Frontiers in plant science · 2020Review
- Functional Similarity and Difference amongGenes · 2019Article
- Epigenetic Regulation of Plant Gametophyte Development.International journal of molecular sciences · 2019Review
- Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics.Plant physiology · 2018Article
- Non-coding RNAs and plant male sterility: current knowledge and future prospects.Plant cell reports · 2018Review
- Small RNA and Degradome Deep Sequencing Reveals the Roles of microRNAs in Seed Expansion in Peanut (Frontiers in plant science · 2018Article
- Loss of function of Arabidopsis microRNA-machinery genes impairs fertility, and has effects on homologous recombination and meiotic chromatin dynamics.Scientific reports · 2017Article
- Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects inThe Plant cell · 2017Article
- Abiotic stress miRNomes in the Triticeae.Functional & integrative genomics · 2017Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundNew generation sequencing technology has allowed investigation of the small RNA populations of flowering plants at great depth. However, little is known about small RNAs in their reproductive cells, especially in post-meiotic cells of the gametophyte generation. Pollen - the male gametophyte - is the specialised haploid structure that generates and delivers the sperm cells to the female gametes at fertilisation. Whether development and differentiation of the male gametophyte depends on the action of microRNAs and trans-acting siRNAs guiding changes in gene expression is largely unknown. Here we have used 454 sequencing to survey the various small RNA populations present in mature pollen of Arabidopsis thaliana.
resultsIn this study we detected the presence of 33 different microRNA families in mature pollen and validated the expression levels of 17 selected miRNAs by Q-RT-PCR. The majority of the selected miRNAs showed pollen-enriched expression compared with leaves. Furthermore, we report for the first time the presence of trans-acting siRNAs in pollen. In addition to describing new patterns of expression for known small RNAs in each of these classes, we identified 7 putative novel microRNAs. One of these, ath-MIR2939, targets a pollen-specific F-box transcript and we demonstrate cleavage of its target mRNA in mature pollen.
conclusionsDespite the apparent simplicity of the male gametophyte, comprising just two different cell types, pollen not only utilises many miRNAs and trans-acting siRNAs expressed in the somatic tissues but also expresses novel miRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.