ArticlePlanta2010
High expression level of a foot and mouth disease virus epitope in tobacco transplastomic plants.
Article in Planta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Homecoming: rewinding the reductive evolution of the chloroplast genome for increasing crop yields.Nature communications · 2021Article
- The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice.Biotechnology letters · 2020Article
- Translocation from the chloroplast stroma into the thylakoid lumen allows expression of recombinant epidermal growth factor in transplastomic tobacco plants.Transgenic research · 2020Article
- Functional Improvement of Human Cardiotrophin 1 Produced in Tobacco Chloroplasts by Co-expression with Plastid Thioredoxin m.Plants (Basel, Switzerland) · 2020Article
- Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials.Scientific reports · 2019Article
- High-level production of single chain monellin mutants with enhanced sweetness and stability in tobacco chloroplasts.Planta · 2018Article
- Plant-based oral vaccines against zoonotic and non-zoonotic diseases.Plant biotechnology journal · 2016Review
- High-level expression of thermostable cellulolytic enzymes in tobacco transplastomic plants and their use in hydrolysis of an industrially pretreated Arundo donax L. biomass.Biotechnology for biofuels · 2016Article
- Expression of the Multimeric and Highly Immunogenic Brucella spp. Lumazine Synthase Fused to Bovine Rotavirus VP8d as a Scaffold for Antigen Production in Tobacco Chloroplasts.Frontiers in plant science · 2015Article
- Article
- Translational fusion and redirection to thylakoid lumen as strategies to enhance accumulation of human papillomavirus E7 antigen in tobacco chloroplasts.Molecular biotechnology · 2014Article
- Production of functional native human interleukin-2 in tobacco chloroplasts.Molecular biotechnology · 2014Article
- Article
- Article
- Article
- The use of plants for the production of therapeutic human peptides.Plant cell reports · 2012Review
- Plant plastid engineering.Current genomics · 2010Article
- Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast transformation has an extraordinary potential for antigen production in plants because of the capacity to accumulate high levels of recombinant proteins and increased biosafety due to maternal plastid inheritance in most crops. In this article, we evaluate tobacco chloroplasts transformation for the production of a highly immunogenic epitope containing amino acid residues 135-160 of the structural protein VP1 of the foot and mouth disease virus (FMDV). To increase the accumulation levels, the peptide was expressed as a fusion protein with the beta-glucuronidase reporter gene (uidA). The recombinant protein represented the 51% of the total soluble proteins in mature leaves, a level higher than those of the Rubisco large subunit, the most abundant protein in the leaf of a wild-type plant. Despite this high accumulation of heterologous protein, the transplastomic plants and wild-type tobacco were phenotypically indistinguishable. The FMDV epitope expressed in transplastomic plants was immunogenic in mice. These results show that transplastomic tobacco express efficiently the recombinant protein, and we conclude that this technology allows the production of large quantities of immunogenic proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.