ArticleThe Journal of biological chemistry2010
Recombinant human collagen XV regulates cell adhesion and migration.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Decoding the Collagenome in Breast Cancer: Mechanotransduction, Microenvironment, and Translational Opportunities.International journal of molecular sciences · 2026Review
- Collagen XV preserves heart function and protects from pathological remodelling after myocardial infarction.The FEBS journal · 2026Article
- The Duality of Collagens in Metastases of Solid Tumors.International journal of molecular sciences · 2025Review
- Extracellular matrix dynamics in tumor immunoregulation: from tumor microenvironment to immunotherapy.Journal of hematology & oncology · 2025Review
- External Scaffold for Strengthening the Pulmonary Autograft in the Ross Procedure.Biomimetics (Basel, Switzerland) · 2024Review
- Dual topologies of myotomal collagen XV and Tenascin C act in concert to guide and shape developing motor axons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Fine-scale mapping of chromosome 9q22.33 identifies candidate causal variant in ovarian cancer.PeerJ · 2024Article
- A ten long noncoding RNA-based prognostic risk model construction and mechanism study in the basal-like immune-suppressed subtype of triple-negative breast cancer.Translational cancer research · 2023Article
- Senescent fibro-adipogenic progenitors are potential drivers of pathology in inclusion body myositis.Acta neuropathologica · 2023Article
- Collagen XV mediated the epithelial-mesenchymal transition to inhibit hepatocellular carcinoma metastasis.Journal of gastrointestinal oncology · 2022Article
- Endocardial/endothelial angiocrines regulate cardiomyocyte development and maturation and induce features of ventricular non-compaction.European heart journal · 2021Article
- Deletion ofInternational journal of molecular sciences · 2021Article
- The Collagens DPY-17 and SQT-3 Direct Anterior-Posterior Migration of the Q Neuroblasts inJournal of developmental biology · 2021Article
- Collagen biology making inroads into prognosis and treatment of cancer progression and metastasis.Cancer metastasis reviews · 2020Review
- Collagen XV, a multifaceted multiplexin present across tissues and species.Matrix biology plus · 2020Article
- The role of collagen in cancer: from bench to bedside.Journal of translational medicine · 2019Review
- Slow Muscle Precursors Lay Down a Collagen XV Matrix Fingerprint to Guide Motor Axon Navigation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Basement membrane zone collagens XV and XVIII/proteoglycans mediate leukocyte influx in renal ischemia/reperfusion.PloS one · 2014Article
- Epigenetic regulation of COL15A1 in smooth muscle cell replicative aging and atherosclerosis.Human molecular genetics · 2013Article
- Tumor suppression by collagen XV is independent of the restin domain.Matrix biology : journal of the International Society for Matrix Biology · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The C-terminal end of collagen XV, restin, has been the focus of several studies, but the functions of full-length collagen XV have remained unknown. We describe here studies on the production, purification, and function of collagen XV and the production of a monoclonal N-terminal antibody to it. Full-length human collagen XV was produced in insect cells using baculoviruses and purified from the cell culture medium. The yield was 15 mg/liter of cell culture medium. The collagen XV was shown to be trimeric, with disulfide bonds in the collagenous region. Rotary shadowing electron microscopy revealed rod-like molecules with a mean length of 241.8 nm and with a globular domain at one end. The globular domain was verified to be the N-terminal end by N-terminal antibody binding. The molecules show flexibility in their conformation, presumably due to the many interruptions in their collagenous domains. The ability of collagen XV to serve as a substrate for cells was tested in cell adhesion assays, and it was shown that cells did not bind to collagen XV-coated surfaces. When added to the culture medium of fibroblasts and fibrosarcoma cells, however, collagen XV rapidly bound to their fibronectin network. Solid phase assays showed that collagen XV binds to fibronectin, laminin, and vitronectin and that it binds to the collagen/gelatin-binding domain of fibronectin. No binding was detected to fibrillar collagens, fibril-associated collagens, or decorin. Interestingly, collagen XV was found to inhibit the adhesion and migration of fibrosarcoma cells when present in fibronectin-containing matrices.
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