ArticleThe Journal of biological chemistry2010
Direct observation and quantitative analysis of Lck exchange between plasma membrane and cytosol in living T cells.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Palmitoylation-dependent regulation of innate and adaptive immunity: molecular insights and translational opportunities.Cellular & molecular biology letters · 2026Review
- UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemia.Life science alliance · 2025Article
- Bayesian metamodeling of early T-cell antigen receptor signaling accounts for its nanoscale activation patterns.Frontiers in immunology · 2024Article
- Optical sensing and control of T cell signaling pathways.Frontiers in physiology · 2023Review
- Regulation of T cell function by protein S-acylation.Frontiers in physiology · 2022Review
- T cell receptor (TCR) signaling in health and disease.Signal transduction and targeted therapy · 2021Review
- Lck bound to coreceptor is less active than free Lck.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Conformational States Control Lck Switching between Free and Confined Diffusion Modes in T Cells.Biophysical journal · 2020Article
- Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells.Frontiers in immunology · 2020Review
- A Fluorescent Kinase Inhibitor that Exhibits Diagnostic Changes in Emission upon Binding.Angewandte Chemie (International ed. in English) · 2019Article
- The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.Developmental cell · 2018Article
- Measuring membrane association and protein diffusion within membranes with supercritical angle fluorescence microscopy.Biomedical optics express · 2016Article
- Tracking single serotonin transporter molecules at the endoplasmic reticulum and plasma membrane.Biophysical journal · 2014Article
- Cross talk between CD3 and CD28 is spatially modulated by protein lateral mobility.Molecular and cellular biology · 2014Article
- Article
- Emerging roles for protein S-palmitoylation in immunity from chemical proteomics.Current opinion in chemical biology · 2013Review
- Amino-terminal cysteine residues differentially influence RGS4 protein plasma membrane targeting, intracellular trafficking, and function.The Journal of biological chemistry · 2012Article
- Targeting protein lipidation in disease.Trends in molecular medicine · 2012Review
- Review
- Lateral dynamics of proteins with polybasic domain on anionic membranes: a dynamic Monte-Carlo study.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Palmitoylation represents a common motif for anchorage of cytosolic proteins to the plasma membrane. Being reversible, it allows for controlled exchange between cytosolic and plasma membrane-bound subpopulations. In this study, we present a live cell single molecule approach for quantifying the exchange kinetics of plasma membrane and cytosolic populations of fluorescently labeled Lck, the key Src family kinase involved in early T cell signaling. Total internal reflection (TIR) fluorescence microscopy was employed for confining the analysis to membrane-proximal molecules. Upon photobleaching Lck-YFP in TIR configuration, fluorescence recovery proceeds first via the cytosol outside of the evanescent field, so that in the early phase fluorescence signal arises predominantly from membrane-proximal cytosolic Lck. The diffusion constant of each molecule allowed us to distinguish whether the molecule has already associated with the plasma membrane or was still freely diffusing in the cytosol. From the number of molecules that inserted during the recovery time we quantified the insertion kinetics: on average, membrane-proximal molecules within the evanescent field needed approximately 400 ms to be inserted. The average lifetime of Lck in the plasma membrane was estimated at 50 s; together with the mobility of 0.26 microm(2)/s this provides sufficient time to explore the surface of the whole T cell before dissociation into the cytosol. Experiments on palmitoylation-deficient Lck mutants yielded similar on-rates, but substantially increased off-rates. We discuss our findings based on a model for the plasma membrane association and dissociation kinetics of Lck, which accounts for reversible palmitoylation on cysteine 3 and 5.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.