ArticleRNA (New York, N.Y.)2010
Preferential translation of Hsp83 in Leishmania requires a thermosensitive polypyrimidine-rich element in the 3' UTR and involves scanning of the 5' UTR.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- Five-layer systems analysis ofeLife · 2026Article
- Queuosine tRNA modification regulates translational adaptation and virulence of Leishmania mexicana.PLoS biology · 2026Article
- Article
- Translational reprogramming under heat stress: a plant's perspective.Royal Society open science · 2025Review
- The role of structure in regulatory RNA elements.Bioscience reports · 2024Review
- Evolutionary divergent clusters of transcribed extinct truncated retroposons drive low mRNA expression and developmental regulation in the protozoan Leishmania.BMC biology · 2024Article
- RNA structure mediated thermoregulation: What can we learn from plants?Frontiers in plant science · 2022Review
- Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring CyanobacteriumFrontiers in microbiology · 2022Article
- Insights intoVeterinary sciences · 2021Review
- Casein kinase 1.2 over expression restores stress resistance to Leishmania donovani HSP23 null mutants.Scientific reports · 2020Article
- An RNA thermoswitch regulates daytime growth in Arabidopsis.Nature plants · 2020Article
- Article
- Deletion of a Single LeishIF4E-3 Allele by the CRISPR-Cas9 System Alters Cell Morphology and Infectivity ofmSphere · 2019Article
- The mRNA-bound Proteome ofMolecular & cellular proteomics : MCP · 2019Article
- Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major.Scientific reports · 2019Article
- Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation.Scientific reports · 2019Article
- Regulation ofmSphere · 2018Article
- Comparative genomics of Tunisian Leishmania major isolates causing human cutaneous leishmaniasis with contrasting clinical severity.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2017Article
- Untranslated regions of mRNA and their role in regulation of gene expression in protozoan parasites.Journal of biosciences · 2017Review
- Species- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids.Genome biology and evolution · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock proteins (HSPs) provide a useful system for studying developmental patterns in the digenetic Leishmania parasites, since their expression is induced in the mammalian life form. Translation regulation plays a key role in control of protein coding genes in trypanosomatids, and is directed exclusively by elements in the 3' untranslated region (UTR). Using sequential deletions of the Leishmania Hsp83 3' UTR (888 nucleotides [nt]), we mapped a region of 150 nt that was required, but not sufficient for preferential translation of a reporter gene at mammalian-like temperatures, suggesting that changes in RNA structure could be involved. An advanced bioinformatics package for prediction of RNA folding (UNAfold) marked the regulatory region on a highly probable structural arm that includes a polypyrimidine tract (PPT). Mutagenesis of this PPT abrogated completely preferential translation of the fused reporter gene. Furthermore, temperature elevation caused the regulatory region to melt more extensively than the same region that lacked the PPT. We propose that at elevated temperatures the regulatory element in the 3' UTR is more accessible to mediators that promote its interaction with the basal translation components at the 5' end during mRNA circularization. Translation initiation of Hsp83 at all temperatures appears to proceed via scanning of the 5' UTR, since a hairpin structure abolishes expression of a fused reporter gene.
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