ArticleThe Plant cell2009
DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 244 papers.
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244 citing papers in PubMed, 557 citations in OpenAlex.
- A hickory diacylglycerol acyltransferase gene coordinates fatty acid metabolism and aluminum tolerance in plants.The New phytologist · 2026Article
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- The chromosome-level genomes of twoHorticulture research · 2026Article
- Opposite diacylglycerol enantiomeric specificities of Arabidopsis DGAT1 and DGAT2 reveal distinct roles in TAG synthesis.Plant physiology · 2026Article
- Oil as a Hindrance to Oat (Foods (Basel, Switzerland) · 2026Review
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Design of High-Diacylglycerol and Lecithin Soybean Seed Oil Using GmPDATs and GmDGATs Knockout via a CRISPR-Cas9 System.Plant biotechnology journal · 2026Article
- Lipid regulation inFrontiers in plant science · 2026Review
- Transcriptome-based analysis of oil accumulation pattern and key gene screening inFrontiers in plant science · 2026Article
- Functional diversity of sunflower DGAT1 and DGAT2 enzymes underlying seed triacylglycerol biosynthesis.Frontiers in plant science · 2026Article
- Characterization ofPlants (Basel, Switzerland) · 2025Article
- Bridging the gaps: advanced techniques to unlock lipid function in plant reproductive development.Plant reproduction · 2025Review
- Complete replacement of Arabidopsis oil-producing enzymes with heterologous diacylglycerol acyltransferases.Plant physiology · 2025Article
- Meta-QTL analysis for mining key genes associated with seed oil content in maize.BMC plant biology · 2025Article
- Characterization of the SoybeanPlants (Basel, Switzerland) · 2025Article
- Telomere-to-telomere genome assembly of linseed (Linum usitatissimum L.) for functional genomics and accelerated genetic improvement.Plant biotechnology journal · 2025Article
- Genetic basis for broad interspecific compatibility in Solanum verrucosum.The Plant journal : for cell and molecular biology · 2025Article
- The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus.Plant cell reports · 2025Article
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184 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triacylglycerol (TAG) biosynthesis is a principal metabolic pathway in most organisms, and TAG is the major form of carbon storage in many plant seeds. Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) is the only acyltransferase enzyme that has been confirmed to contribute to TAG biosynthesis in Arabidopsis thaliana seeds. However, dgat1 null mutants display only a 20 to 40% decrease in seed oil content. To determine whether other enzymes contribute to TAG synthesis, candidate genes were expressed in TAG-deficient yeast, candidate mutants were crossed with the dgat1-1 mutant, and target genes were suppressed by RNA interference (RNAi). An in vivo role for phospholipid:diacylglycerol acyltransferase 1 (PDAT1; At5g13640) in TAG synthesis was revealed in this study. After failing to obtain double homozygous plants from crossing dgat1-1 and pdat1-2, further investigation showed that the dgat1-1 pdat1-2 double mutation resulted in sterile pollen that lacked visible oil bodies. RNAi silencing of PDAT1 in a dgat1-1 background or DGAT1 in pdat1-1 background resulted in 70 to 80% decreases in oil content per seed and in disruptions of embryo development. These results establish in vivo involvement of PDAT1 in TAG biosynthesis, rule out major contributions by other candidate enzymes, and indicate that PDAT1 and DGAT1 have overlapping functions that are essential for normal pollen and seed development of Arabidopsis.
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