ArticleEvidence-based complementary and alternative medicine : eCAM2011
RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3K/Akt Signaling Mediated by IGF-I.
Article in Evidence-based complementary and alternative medicine : eCAM, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Isolation and Characterization of Earthworm Peptides with Neuroprotective Effects in Parkinson's Disease Models.Molecules (Basel, Switzerland) · 2025Article
- Pharmacological effects of bioactive agents in earthworm extract: A comprehensive review.Animal models and experimental medicine · 2024Review
- Decoding the mechanism of earthworm extract against wounds: an integrated metabolomics and network pharmacology study.Molecular diversity · 2024Article
- Baicalin promotes the viability of Schwann cellsExperimental and therapeutic medicine · 2017Article
- The Proliferation Enhancing Effects of Salidroside on Schwann Cells In Vitro.Evidence-based complementary and alternative medicine : eCAM · 2017Article
- Up-regulation of NF45 correlates with Schwann cell proliferation after sciatic nerve crush.Journal of molecular neuroscience : MN · 2015Article
- Earthworm (Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation.BMC complementary and alternative medicine · 2014Article
- Neuroprotective effects of Buyang Huanwu decoction on cerebral ischemia-induced neuronal damage.Neural regeneration research · 2014Article
- Origin of innate immune responses: revelation of food and medicinal applications.Journal of traditional and complementary medicine · 2013Review
- Dilong: food for thought and medicine.Journal of traditional and complementary medicine · 2012Article
- Effect of oral administration of Pheretima aspergillum (earthworm) in rats with cerebral infarction induced by middle-cerebral artery occlusion.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2012Article
- Earthworms dilong: ancient, inexpensive, noncontroversial models may help clarify approaches to integrated medicine emphasizing neuroimmune systems.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- Dilong: role in peripheral nerve regeneration.Evidence-based complementary and alternative medicine : eCAM · 2011Article
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Schwann cell proliferation is critical for the regeneration of injured nerves. Dilongs are widely used in Chinese herbal medicine to remove stasis and stimulate wound-healing functions. Exactly how this Chinese herbal medicine promotes tissue survival remains unclear. The aim of the present study was to investigate the molecular mechanisms by which Dilong promote neuron regeneration. Our results show that treatment with extract of Dilong induces the phosphorylation of the insulin-like growth factor-I (IGF-I)-mediated phosphatidylinositol 3-kinase/serine-threonine kinase (PI3K/Akt) pathway, and activates protein expression of cell nuclear antigen (PCNA) in a time-dependent manner. Cell cycle analysis showed that G(1) transits into the S phase in 12-16 h, and S transits into the G(2) phase 20 h after exposure to earthworm extract. Strong expression of cyclin D1, cyclin E and cyclin A occurs in a time-dependent manner. Small interfering RNA (siRNA)-mediated knockdown of PI3K significantly reduced PI3K protein expression levels, resulting in Bcl(2) survival factor reduction and a marked blockage of G(1) to S transition in proliferating cells. These results demonstrate that Dilong promotes the proliferation and survival of RSC96 cells via IGF-I signaling. The mechanism is mainly dependent on the PI3K protein.
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