ArticleBMC evolutionary biology2009
Identification and dynamics of a beneficial mutation in a long-term evolution experiment with Escherichia coli.
Article in BMC evolutionary biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Revisiting the Design of the Long-Term Evolution Experiment with Escherichia coli.Journal of molecular evolution · 2023Review
- Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors.Microorganisms · 2023Article
- Extracellular Vesicles from 50,000 Generation Clones of theInternational journal of molecular sciences · 2022Article
- Quantum aspects of evolution: a contribution towards evolutionary explorations of genotype networks via quantum walks.Journal of the Royal Society, Interface · 2020Article
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- What is adaptation by natural selection? Perspectives of an experimental microbiologist.PLoS genetics · 2017Review
- Inter-laboratory evolution of a model organism and its epistatic effects on mutagenesis screens.Scientific reports · 2016Article
- Evolutionary dynamics of morphological stability in a long-term experiment with Escherichia coli.IET systems biology · 2015Article
- Directed evolution of cell size in Escherichia coli.BMC evolutionary biology · 2014Article
- A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.BMC biotechnology · 2014Article
- Detecting rare structural variation in evolving microbial populations from new sequence junctions using breseq.Frontiers in genetics · 2014Article
- Are molecular alphabets universal enabling factors for the evolution of complex life?Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2013Article
- Genome duplication and mutations in ACE2 cause multicellular, fast-sedimenting phenotypes in evolved Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The environment affects epistatic interactions to alter the topology of an empirical fitness landscape.PLoS genetics · 2013Article
- Article
- Historical contingency affects signaling strategies and competitive abilities in evolving populations of simulated robots.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Molecular spandrels: tests of adaptation at the genetic level.Nature reviews. Genetics · 2011Review
- Hypermutation and stress adaptation in bacteria.Journal of genetics · 2011Review
- Second-order selection for evolvability in a large Escherichia coli population.Science (New York, N.Y.) · 2011Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTwelve populations of E. coli were serially propagated for 20,000 generations in a glucose-supplemented minimal medium in order to study the dynamics of evolution. We sought to find and characterize one of the beneficial mutations responsible for the adaptation and other phenotypic changes, including increased cell size, in one of these populations.
resultsWe used transposon-tagging followed by P1-transduction into the ancestor, screening for increased cell size and fitness, co-transduction analysis, and DNA sequencing. We identified a 1-bp insertion in the BoxG1 region located upstream of glmUS, an operon involved in cell-wall biosynthesis. When transduced into the ancestor, this mutation increased competitive fitness by about 5%. This mutation spread through its population of origin between 500 and 1500 generations. Mutations in this region were not found in the other 11 evolving populations, even after 20,000 generations.
conclusionThe 1-bp insertion in the BoxG1 region near glmUS was demonstrably beneficial in the environment in which it arose. The absence of similar mutations in the other evolved populations suggests that they substituted other mutations that rendered this particular mutation unimportant. These results show the unpredictability of adaptive evolution, whereas parallel substitutions at other loci in these same populations reveal the predictability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.