ArticlePhotosynthesis research2010
Enzymatic properties of the ferredoxin-dependent nitrite reductase from Chlamydomonas reinhardtii. Evidence for hydroxylamine as a late intermediate in ammonia production.
Article in Photosynthesis research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- Pathways and mechanisms for efficient nitrogen utilization in the high-temperature tolerant heterotrophic nitrifying bacterium Aeribacillus pallidus sp. GW-E.BMC microbiology · 2025Article
- Identifying Endogenous Proteins of Perennial Ryegrass (Proteomes · 2025Article
- Electrochemical nitrate reduction in acid enables high-efficiency ammonia synthesis and high-voltage pollutes-based fuel cells.Nature communications · 2023Article
- Redox Regulation of Salt Tolerance inInternational journal of molecular sciences · 2023Article
- Role of distal arginine residue in the mechanism of heme nitrite reductases.Chemical science · 2023Article
- Integrating brucine with carbon nanotubes toward electrochemical sensing of hydroxylamine.Mikrochimica acta · 2020Article
- Bicarbonate Induced Redox Proteome Changes in Arabidopsis Suspension Cells.Frontiers in plant science · 2017Article
- Remembering David B. Knaff (1941-2016).Photosynthesis research · 2016Article
- The Involvement of hybrid cluster protein 4, HCP4, in Anaerobic Metabolism in Chlamydomonas reinhardtii.PloS one · 2016Article
- Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism.The Plant cell · 2014Article
- Heme-bound nitroxyl, hydroxylamine, and ammonia ligands as intermediates in the reaction cycle of cytochrome c nitrite reductase: a theoretical study.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
- Nitrate ammonification by Nautilia profundicola AmH: experimental evidence consistent with a free hydroxylamine intermediate.Frontiers in microbiology · 2013Article
- Nitrite biosensing via selective enzymes--a long but promising route.Sensors (Basel, Switzerland) · 2010Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
The ferredoxin-dependent nitrite reductase from the green alga Chlamydomonas reinhardtii has been cloned, expressed in Escherichia coli as a His-tagged recombinant protein, and purified to homogeneity. The spectra, kinetic properties and substrate-binding parameters of the C. reinhardtii enzyme are quite similar to those of the ferredoxin-dependent spinach chloroplast nitrite reductase. Computer modeling, based on the published structure of spinach nitrite reductase, predicts that the structure of C. reinhardtii nitrite reductase will be similar to that of the spinach enzyme. Chemical modification studies and the ionic-strength dependence of the enzyme's ability to interact with ferredoxin are consistent with the involvement of arginine and lysine residues on C. reinhardtii nitrite reductase in electrostatically-stabilized binding to ferredoxin. The C. reinhardtii enzyme has been used to demonstrate that hydroxylamine can serve as an electron-accepting substrate for the enzyme and that the product of hydroxylamine reduction is ammonia, providing the first experimental evidence for the hypothesis that hydroxylamine, bound to the enzyme, can serve as a late intermediate during the reduction of nitrite to ammonia catalyzed by the enzyme.
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Registered trials
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