ArticleApplied and environmental microbiology2010
Genes involved in yellow pigmentation of Cronobacter sakazakii ES5 and influence of pigmentation on persistence and growth under environmental stress.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 56 citations in OpenAlex.
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- A New Insight into the Bactericidal Mechanism of 405 nm Blue Light-Emitting-Diode against Dairy SourcedFoods (Basel, Switzerland) · 2021Article
- Genome Insights of the Plant-Growth Promoting BacteriumFrontiers in microbiology · 2020Article
- Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in FoodborneFrontiers in microbiology · 2019Article
- Xerotolerant bacteria: surviving through a dry spell.Nature reviews. Microbiology · 2017Review
- Screening of genes involved in interactions with intestinal epithelial cells in Cronobacter sakazakii.AMB Express · 2016Article
- Adaptation of Staphylococcus xylosus to Nutrients and Osmotic Stress in a Salted Meat Model.Frontiers in microbiology · 2016Article
- Reconstruction of the carotenoid biosynthetic pathway of Cronobacter sakazakii BAA894 in Escherichia coli.PloS one · 2014Article
- Identification of genes involved in serum tolerance in the clinical strain Cronobacter sakazakii ES5.BMC microbiology · 2013Article
- Biological role of pigment production for the bacterial phytopathogen Pantoea stewartii subsp. stewartii.Applied and environmental microbiology · 2012Article
- Polymorphisms in rpoS and stress tolerance heterogeneity in natural isolates of Cronobacter sakazakii.Applied and environmental microbiology · 2012Article
- Comprehensive approaches to molecular biomarker discovery for detection and identification of Cronobacter spp. (Enterobacter sakazakii) and Salmonella spp.Applied and environmental microbiology · 2011Article
- Genes involved in Cronobacter sakazakii biofilm formation.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cronobacter spp. are opportunistic food-borne pathogens that are responsible for rare but highly fatal cases of meningitis and necrotizing enterocolitis in neonates. While the operon responsible for yellow pigmentation in Cronobacter sakazakii strain ES5 was described recently, the involvement of additional genes in pigment expression and the influence of pigmentation on the fitness of Cronobacter spp. have not been investigated. Thus, the aim of this study was to identify further genes involved in pigment expression in Cronobacter sakazakii ES5 and to assess the influence of pigmentation on growth and persistence under conditions of environmental stress. A knockout library was created using random transposon mutagenesis. The screening of 9,500 mutants for decreased pigment production identified 30 colorless mutants. The mapping of transposon insertion sites revealed insertions in not only the carotenoid operon but also in various other genes involved in signal transduction, inorganic ions, and energy metabolism. To determine the effect of pigmentation on fitness, colorless mutants (DeltacrtE, DeltacrtX, and DeltacrtY) were compared to the yellow wild type using growth and inactivation experiments, a macrophage assay, and a phenotype array. Among other findings, the colorless mutants grew at significantly increased rates under osmotic stress compared to that of the yellow wild type while showing increased susceptibility to desiccation. Moreover, DeltacrtE and DeltacrtY exhibited increased sensitivity to UVB irradiation.
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