ArticleApplied and environmental microbiology2010
Identification of a two-component regulatory pathway essential for Mn(II) oxidation in Pseudomonas putida GB-1.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Identification of Molecular Pathways Underlying Mn(II) Oxidation in Pseudomonas resinovorans.Environmental microbiology · 2026Article
- Article
- Population-level control of two manganese oxidases expands the niche for bacterial manganese biomineralization.NPJ biofilms and microbiomes · 2025Article
- Isolation, characterization, and genetic manipulation of cold-tolerant, manganese-oxidizingApplied and environmental microbiology · 2024Article
- Advances in Research on Bacterial Oxidation of Mn(II): A Visualized Bibliometric Analysis Based on CiteSpace.Microorganisms · 2024Review
- Cyclic di-GMP Signaling Links Biofilm Formation and Mn(II) Oxidation in Pseudomonas resinovorans.mBio · 2022Article
- Genome analysis of Pseudomonas sp. OF001 and Rubrivivax sp. A210 suggests multicopper oxidases catalyze manganese oxidation required for cylindrospermopsin transformation.BMC genomics · 2021Article
- Removal of Mn(II) by a nitrifying bacterium Acinetobacter sp. AL-6: efficiency and mechanisms.Environmental science and pollution research international · 2021Article
- Mechanisms of Manganese(II) Oxidation by Filamentous Ascomycete Fungi Vary With Species and Time as a Function of Secretome Composition.Frontiers in microbiology · 2021Article
- Anti-bacterial Effects of MnOMicrobes and environments · 2020Article
- Draft Genome Sequence of Pseudomonas sp. Strain LLC-1 (NBRC 111237), Capable of Metabolizing Lignin-Derived Low-Molecular-Weight Compounds.Genome announcements · 2018Article
- Mechanistic insights into manganese oxidation of a soil-borne Mn(II)-oxidizing Escherichia coli strain by global proteomic and genetic analyses.Scientific reports · 2017Article
- Article
- Identification of a Third Mn(II) Oxidase Enzyme in Pseudomonas putida GB-1.Applied and environmental microbiology · 2016Article
- Surface Mn(II) oxidation actuated by a multicopper oxidase in a soil bacterium leads to the formation of manganese oxide minerals.Scientific reports · 2015Article
- An intimate link: two-component signal transduction systems and metal transport systems in bacteria.Future microbiology · 2014Review
- Genome features of Pseudomonas putida LS46, a novel polyhydroxyalkanoate producer and its comparison with other P. putida strains.AMB Express · 2014Article
- Effects of exogenous pyoverdines on Fe availability and their impacts on Mn(II) oxidation by Pseudomonas putida GB-1.Frontiers in microbiology · 2014Article
- Pyoverdine synthesis by the Mn(II)-oxidizing bacterium Pseudomonas putida GB-1.Frontiers in microbiology · 2014Article
- A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis.Nature chemical biology · 2013Article
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2 authors.
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Abstract
Bacterial manganese(II) oxidation has a profound impact on the biogeochemical cycling of Mn and the availability of the trace metals adsorbed to the surfaces of solid Mn(III, IV) oxides. The Mn(II) oxidase enzyme was tentatively identified in Pseudomonas putida GB-1 via transposon mutagenesis: the mutant strain GB-1-007, which fails to oxidize Mn(II), harbors a transposon insertion in the gene cumA. cumA encodes a putative multicopper oxidase (MCO), a class of enzymes implicated in Mn(II) oxidation in other bacterial species. However, we show here that an in-frame deletion of cumA did not affect Mn(II) oxidation. Through complementation analysis of the oxidation defect in GB-1-007 with a cosmid library and subsequent sequencing of candidate genes we show the causative mutation to be a frameshift within the mnxS1 gene that encodes a putative sensor histidine kinase. The frameshift mutation results in a truncated protein lacking the kinase domain. Multicopy expression of mnxS1 restored Mn(II) oxidation to GB-1-007 and in-frame deletion of mnxS1 resulted in a loss of oxidation in the wild-type strain. These results clearly demonstrated that the oxidation defect of GB-1-007 is due to disruption of mnxS1, not cumA::Tn5, and that CumA is not the Mn(II) oxidase. mnxS1 is located upstream of a second sensor histidine kinase gene, mnxS2, and a response regulator gene, mnxR. In-frame deletions of each of these genes also led to the loss of Mn(II) oxidation. Therefore, we conclude that the MnxS1/MnxS2/MnxR two-component regulatory pathway is essential for Mn(II) oxidation in P. putida GB-1.
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