ArticleJournal of immunology (Baltimore, Md. : 1950)2010
Defective ribosomal products are the major source of antigenic peptides endogenously generated from influenza A virus neuraminidase.
Article in Journal of immunology (Baltimore, Md. : 1950), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Beyond Sequence: Posttranslational Remodeling of Antigens in Autoimmunity.Immunological reviews · 2026Review
- Neuraminidase delivered as an APC-targeted DNA vaccine induces protective antibodies against influenza.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Know thy immune self and non-self: Proteomics informs on the expanse of self and non-self, and how and where they arise.Proteomics · 2021Review
- Review
- Surprisingly Effective Priming of CD8Journal of virology · 2020Article
- Article
- Quantification of epitope abundance reveals the effect of direct and cross-presentation on influenza CTL responses.Nature communications · 2019Article
- Flu DRiPs in MHC Class I Immunosurveillance.Virologica Sinica · 2019Review
- Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance.Molecular cell · 2019Article
- Influenza A Virus Negative Strand RNA Is Translated for CD8Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells.Immune network · 2018Article
- Alternative Start Sites Downstream of Non-Sense Mutations Drive Antigen Presentation and Tolerance Induction to C-Terminal Epitopes.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Different Polyubiquitinated Bodies in Human Dendritic Cells: IL-4 Causes PaCS During Differentiation while LPS or IFNα Induces DALIS During Maturation.Scientific reports · 2017Article
- Defining Viral Defective Ribosomal Products: Standard and Alternative Translation Initiation Events Generate a Common Peptide from Influenza A Virus M2 and M1 mRNAs.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Enhanced Recognition of HIV-1 Cryptic Epitopes Restricted by HLA Class I Alleles Associated With a Favorable Clinical Outcome.Journal of acquired immune deficiency syndromes (1999) · 2015Article
- Ubiquitous Autofragmentation of Fluorescent Proteins Creates Abundant Defective Ribosomal Products (DRiPs) for Immunosurveillance.The Journal of biological chemistry · 2015Article
- Biogenesis of influenza a virus hemagglutinin cross-protective stem epitopes.PLoS pathogens · 2014Article
- Apoptotic blebs from leukemic cells as a preferred source of tumor-associated antigen for dendritic cell-based vaccines.Cancer immunology, immunotherapy : CII · 2014Article
- Characterization of T-cell responses to cryptic epitopes in recipients of a noncodon-optimized HIV-1 vaccine.Journal of acquired immune deficiency syndromes (1999) · 2014Article
- Influenza A virus hemagglutinin trimerization completes monomer folding and antigenicity.Journal of virology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The defective ribosomal product (DRiP) hypothesis of endogenous Ag processing posits that rapidly degraded forms of nascent proteins are a major source of peptide ligands for MHC class I molecules. Although there is broad experimental support for the DRiP hypothesis, careful kinetic analysis of the generation of defined peptide class I complexes has been limited to studies of recombinant vaccinia viruses expressing genes derived from other organisms. In this study, we show that insertion of the SIINFEKL peptide into the stalk of influenza A virus neuraminidase (NA) does not detectably modify NA folding, degradation, transport, or sp. act. when expressed in its natural context of influenza A virus infection. Using the 25-D1.16 mAb specific for K(b)-SIINFEKL to precisely quantitate cell surface complexes by flow cytometry, we demonstrate that SIINFEKL is generated in complete lockstep with initiation and abrogation of NA biosynthesis in both L-K(b) fibroblast cells and DC2.4 dendritic/monocyte cells. SIINFEKL presentation requires active proteasomes and TAP, consistent with its generation from a cytosolic DRiP pool. From the difference in the shutoff kinetics of K(b)-SIINFEKL complex expression following protein synthesis versus proteasome inhibition, we estimate that the t(1/2) of the biosynthetic source of NA peptide is approximately 5 min. These observations extend the relevance of the DRiP hypothesis to viral proteins generated in their natural context.
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