ArticleGenetics2010
Alternative splicing modulates Ubx protein function in Drosophila melanogaster.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- The functional evolution of collembolan Ubx on the regulation of abdominal appendage formation.Development genes and evolution · 2024Article
- A Single MicroRNA-Hox Gene Module Controls Equivalent Movements in Biomechanically Distinct Forms of Drosophila.Current biology : CB · 2019Article
- Control of Hox transcription factor concentration and cell-to-cell variability by an auto-regulatory switch.Development (Cambridge, England) · 2019Article
- MicroRNA-dependent regulation of Hox gene expression sculpts fine-grain morphological patterns in aDevelopment (Cambridge, England) · 2018Article
- Combinatorial Gene Regulatory Functions Underlie Ultraconserved Elements in Drosophila.Molecular biology and evolution · 2016Article
- To Be Specific or Not: The Critical Relationship Between Hox And TALE Proteins.Trends in genetics : TIG · 2016Review
- Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox Proteins.The Journal of biological chemistry · 2015Review
- Rethinking gene regulatory networks in light of alternative splicing, intrinsically disordered protein domains, and post-translational modifications.Frontiers in cell and developmental biology · 2015Article
- Article
- Classification of intrinsically disordered regions and proteins.Chemical reviews · 2014Review
- The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.Development (Cambridge, England) · 2014Article
- Onychophoran Hox genes and the evolution of arthropod Hox gene expression.Frontiers in zoology · 2014Article
- Article
- Tissue-specific splicing of a ubiquitously expressed transcription factor is essential for muscle differentiation.Genes & development · 2013Article
- Regulated functional alternative splicing in Drosophila.Nucleic acids research · 2012Review
- Functional synthetic Antennapedia genes and the dual roles of YPWM motif and linker size in transcriptional activation and repression.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Selection of distinct Hox-Extradenticle interaction modes fine-tunes Hox protein activity.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
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Authors and funding
7 authors.
Funding
Abstract
The Drosophila Hox gene Ultrabithorax (Ubx) produces a family of protein isoforms through alternative splicing. Isoforms differ from one another by the presence of optional segments-encoded by individual exons-that modify the distance between the homeodomain and a cofactor-interaction module termed the "YPWM" motif. To investigate the functional implications of Ubx alternative splicing, here we analyze the in vivo effects of the individual Ubx isoforms on the activation of a natural Ubx molecular target, the decapentaplegic (dpp) gene, within the embryonic mesoderm. These experiments show that the Ubx isoforms differ in their abilities to activate dpp in mesodermal tissues during embryogenesis. Furthermore, using a Ubx mutant that reduces the full Ubx protein repertoire to just one single isoform, we obtain specific anomalies affecting the patterning of anterior abdominal muscles, demonstrating that Ubx isoforms are not functionally interchangeable during embryonic mesoderm development. Finally, a series of experiments in vitro reveals that Ubx isoforms also vary in their capacity to bind DNA in presence of the cofactor Extradenticle (Exd). Altogether, our results indicate that the structural changes produced by alternative splicing have functional implications for Ubx protein function in vivo and in vitro. Since other Hox genes also produce splicing isoforms affecting similar protein domains, we suggest that alternative splicing may represent an underestimated regulatory system modulating Hox gene specificity during fly development.
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