ArticleRNA (New York, N.Y.)2010
High-throughput quantification of splicing isoforms.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
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- Transcription factors induce differential splicing of duplicated ribosomal protein genes during meiosis.Nucleic acids research · 2025Article
- IsoPrimer: a pipeline for designing isoform-aware primer pairs for comprehensive gene expression quantification.Bioinformatics advances · 2025Article
- Age-dependent acquisition of pathogenicity by SARS-CoV-2 Omicron BA.5.Science advances · 2023Article
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- PRMT5 inhibition disrupts splicing and stemness in glioblastoma.Nature communications · 2021Article
- Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production.PLoS pathogens · 2020Article
- Telomerase biogenesis requires a novel Mex67 function and a cytoplasmic association with the SmeLife · 2020Article
- Real-time detection of mRNA splicing variants with specifically designed reverse-transcription loop-mediated isothermal amplification.RSC advances · 2020Article
- Effect of Low Versus High Tidal-Volume Total Liquid Ventilation on Pulmonary Inflammation.Frontiers in physiology · 2020Article
- Dichotomic effects of clinically used drugs on tumor growth, bone remodeling and pain management.Scientific reports · 2019Article
- G-quadruplex located in the 5'UTR of the BAG-1 mRNA affects both its cap-dependent and cap-independent translation through global secondary structure maintenance.Nucleic acids research · 2019Article
- miRNA Profiles in Extracellular Vesicles From Serum Early in Pregnancies Complicated by Gestational Diabetes Mellitus.The Journal of clinical endocrinology and metabolism · 2019Article
- Dissecting the expression landscape of cytochromes P450 in hepatocellular carcinoma: towards novel molecular biomarkers.Genes & cancer · 2019Article
- The tyrosine phosphatase Shp-2 confers resistance to colonic inflammation by driving goblet cell function and crypt regeneration.The Journal of pathology · 2019Article
- Spatiotemporal Loss ofCancer discovery · 2019Article
- Simultaneous sequencing of coding and noncoding RNA reveals a human transcriptome dominated by a small number of highly expressed noncoding genes.RNA (New York, N.Y.) · 2018Article
- Transcriptome analysis reveals TMPRSS6 isoforms with distinct functionalities.Journal of cellular and molecular medicine · 2018Article
- G-Quadruplexes influence pri-microRNA processing.RNA biology · 2018Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most human messenger RNAs (mRNAs) are alternatively spliced and many exhibit disease-specific splicing patterns. However, the contribution of most splicing events to the development and maintenance of human diseases remains unclear. As the contribution of alternative splicing events to diagnosis and prognosis is becoming increasingly recognized, it becomes important to develop precise methods to quantify the abundance of these isoforms in clinical samples. Here we present a pipeline for real-time PCR annotation of splicing events (RASE) that allows accurate identification of a large number of splicing isoforms in human tissues. The RASE automatically designed specific primer pairs for 81% of all alternative splicing events in the NCBI build 36 database. Experimentally, the majority of the RASE designed primers resulted in isoform-specific amplification suitable for quantification in human cell lines or in formalin-fixed, paraffin-embedded (FFPE) RNA extract. Using this pipeline it is now possible to rapidly identify splicing isoform signatures in different types of human tissues or to validate complete sets of data generated by microarray expression profiling and deep sequencing techniques.
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