ArticleThe Journal of general physiology2010
Calcium-activated chloride current amplifies the response to urine in mouse vomeronasal sensory neurons.
Article in The Journal of general physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 75 citations in OpenAlex.
- Chemosensory Receptors in Vertebrates: Structure and Computational Modeling Insights.International journal of molecular sciences · 2025Review
- CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Article
- Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal organ at Single Cell Resolution.bioRxiv : the preprint server for biology · 2024Article
- Cyclophosphamide has Long-Term Effects on Proliferation in Olfactory Epithelia.Chemical senses · 2020Article
- Signal Detection and Coding in the Accessory Olfactory System.Chemical senses · 2018Review
- CaThe Journal of biological chemistry · 2018Article
- The long tale of the calcium activated ClChannels (Austin, Tex.) · 2017Review
- Type 3 inositol 1,4,5-trisphosphate receptor is dispensable for sensory activation of the mammalian vomeronasal organ.Scientific reports · 2017Article
- Article
- Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons.Chemical senses · 2016Article
- Expression of calcium-activated chloride channels Ano1 and Ano2 in mouse taste cells.Pflugers Archiv : European journal of physiology · 2016Article
- Intracellular chloride concentration of the mouse vomeronasal neuron.BMC neuroscience · 2015Article
- Conditional knockout of TMEM16A/anoctamin1 abolishes the calcium-activated chloride current in mouse vomeronasal sensory neurons.The Journal of general physiology · 2015Article
- TRICK or TRP? What Trpc2(-/-) mice tell us about vomeronasal organ mediated innate behaviors.Frontiers in neuroscience · 2015Article
- Article
- A wide range of pheromone-stimulated sexual and reproductive behaviors in female mice depend on G protein Gαo.BMC biology · 2014Article
- The genomic basis of vomeronasal-mediated behaviour.Mammalian genome : official journal of the International Mammalian Genome Society · 2014Review
- Calmodulin-dependent activation and inactivation of anoctamin calcium-gated chloride channels.The Journal of general physiology · 2013Article
- Either main or accessory olfactory system signaling can mediate the rewarding effects of estrous female chemosignals in sexually naive male mice.Behavioral neuroscience · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The vomeronasal organ (VNO) is an odor detection system that mediates many pheromone-sensitive behaviors. Vomeronasal sensory neurons (VSNs), located in the VNO, are the initial site of interaction with odors/pheromones. However, how an individual VSN transduces chemical signals into electrical signals is still unresolved. Here, we show that a Ca2+-activated Cl- current contributes approximately 80% of the response to urine in mouse VSNs. Using perforated patch clamp recordings with gramicidin, which leaves intracellular chloride undisrupted, we found that the urine-induced inward current (V(hold) = -80 mV) was decreased in the presence of chloride channel blockers. This was confirmed using whole cell recordings and altering extracellular chloride to shift the reversal potential. Further, the urine-induced currents were eliminated when both extracellular Ca2+ and Na+ were removed. Using inside-out patches from dendritic tips, we recorded Ca2+-activated Cl- channel activity. Several candidates for this Ca2+-activated Cl- channel were detected in VNO by reverse transcription-polymerase chain reaction. In addition, a chloride cotransporter, Na+-K+-2Cl- isoform 1, was detected and found to mediate much of the chloride accumulation in VSNs. Collectively, our data demonstrate that chloride acts as a major amplifier for signal transduction in mouse VSNs. This amplification would increase the responsiveness to pheromones or odorants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.