ArticleJournal of the American Chemical Society2010
MNAzymes, a versatile new class of nucleic acid enzymes that can function as biosensors and molecular switches.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Chemically Evolved XNAzyme Platforms for Effective In Vivo Gene Regulation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Dephosphorylation-regulated MNAzyme-PER cascade as a programmable converter for multi-task molecular actuation.Journal of nanobiotechnology · 2026Article
- Protein-free colorimetric biosensor for sensitive detection of HPV-18 mRNA via MNAzyme-driven catalytic hairpin assembly.Scientific reports · 2026Article
- The 10-23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions.Angewandte Chemie (International ed. in English) · 2026Review
- Chemically Modified DNAzyme with Enhanced Activity for Sensitive MicroRNA Imaging in Live Cells.Molecules (Basel, Switzerland) · 2026Article
- Rational Design Strategies for Stimuli-Responsive DNAzymes Using Modified and Artificial Nucleotides.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Dual-Site Chemically Engineered MNAzymes for One-Pot Isothermal Detection of MicroRNAs.ACS omega · 2025Article
- An Ultra-Sensitive Quarantine Pathogen On-Site Detection Based on a One-Pot Asymmetric Recombinase Polymerase Amplification and MNAzyme-Assisted Target Recycling Biosensor (OAR-MNA).Plant biotechnology journal · 2025Article
- An Intelligent Allosteric DNAzyme Platform for On-Site Uracil-DNA Glycosylase Imaging and On-Demand Gene Regulation.JACS Au · 2025Article
- Enzyme- and DNAzyme-Driven Transient Assembly of DNA-Based Phase-Separated Coacervate Microdroplets.Journal of the American Chemical Society · 2025Article
- Differentiation ofBio-protocol · 2025Article
- Multicomponent nucleic acid enzymes as signal amplification strategy for the detection of microRNA based on fluorescence resonance energy transfer.Mikrochimica acta · 2025Article
- MNAzyme-Assisted Nucleic Acid Lateral Flow Assay for Cost-Effective, On-Site Mercury Detection.Biosensors · 2024Article
- Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach.Biosensors · 2024Article
- Modular CRISPR/Cas12a synergistic activation platform for detection and logic operations.Nucleic acids research · 2024Article
- Engineering Gene-Specific DNAzymes for Accessible and Multiplexed Nucleic Acid Testing.JACS Au · 2024Article
- MNAzyme catalyzed signal amplification-mediated lateral flow biosensor for portable and sensitive detection of mycotoxin in food samples.Analytical and bioanalytical chemistry · 2024Article
- An autocatalytic multicomponent DNAzyme nanomachine for tumor-specific photothermal therapy sensitization in pancreatic cancer.Nature communications · 2023Article
- MNAzymes and gold nanoparticles as isothermal signal amplification strategy for visual detection of miRNA.Mikrochimica acta · 2023Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To increase the versatility and utility of nucleic acid enzymes, we developed multicomponent complexes, known as MNAzymes, which produce amplified "output" signals in response to specific "input" signals. Multiple oligonucleotide partzymes assemble into active MNAzymes only in the presence of an input assembly facilitator such as a target nucleic acid. Once formed, MNAzymes catalytically modify a generic substrate, generating an amplified output signal that heralds the presence of the target while leaving the target intact. We demonstrated several applications including sensitive, isothermal target detection; discrimination of polymorphisms; and highly specific monitoring of real-time polymerase chain reaction (PCR). Furthermore, we showed their capacity to function as molecular switches and to work in series to create a molecular cascade. The modular nature of MNAzymes, together with the separation of input and output functionalities, provides potential for their integration into diverse devices such as diagnostic biosensors, molecular computers, and/or nanoscale machines.
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